Code | CSB-EL022398RA |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 89 | ||||
Range % | 84-92 | |||||
1:2000 | Average % | 95 | ||||
Range % | 91-98 | |||||
1:4000 | Average % | 88 | ||||
Range % | 84-91 | |||||
1:8000 | Average % | 99 | ||||
Range % | 93-102 |
The recovery of rat SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 87-93 | ||||
EDTA plasma (n=4) | 101 | 98-104 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Superoxide dismutase [Mn], mitochondrial (SOD2) ELISA kit is a powerful tool for researchers studying oxidative stress and metabolism in Rattus norvegicus (rat) samples. This ELISA kit is designed to accurately quantify SOD2 levels in serum, plasma, and tissue homogenates.
The Rat SOD2 ELISA kit has a detection range of 1.87 pg/mL-120 pg/mL and a sensitivity of 0.47 pg/mL, making it a highly precise tool for detecting even trace amounts of SOD2 in samples. With an assay time of 1-5 hours and a sample volume requirement of 50-100 ul.
The assay principle of the Rat SOD2 ELISA kit is quantitative and based on a sandwich measurement. The kit utilizes a high-quality antibody specific to SOD2, ensuring accurate and reliable results. The detection wavelength is 450 nm, providing a clear and precise readout of results.
The Rat SOD2 ELISA kit is a critical tool for researchers studying oxidative stress and metabolism. SOD2 plays a critical role in protecting cells from oxidative stress and is essential for maintaining cellular homeostasis. This kit has been specifically designed to accurately measure SOD2 levels in rat samples, making it an essential addition to any lab studying oxidative stress and metabolism.
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