Rat Syndecan-1/CD138(SDC1) ELISA Kit

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Rat Syndecan-1/CD138(SDC1) ELISA Kit

Citations (5) Protocol

Code	CSB-E17115r
Size	96T,5×96T,10×96T How to order?
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Citations (5)
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Description

This Rat SDC1 ELISA Kit was designed for the quantitative measurement of Rat SDC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name

Syndecan-1/CD138(SDC1)

Alternative Names

Sdc1 ELISA Kit; Synd1 ELISA Kit; Syndecan-1 ELISA Kit; SYND1 ELISA Kit; CD antigen CD138 ELISA Kit

Abbreviation

SDC1

Uniprot No.

P26260

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-102
1:2	Average %	101
	Range %	95-104
1:4	Average %	90
	Range %	83-94
1:8	Average %	94
	Range %	87-99

Recovery

The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	94-107
EDTA plasma (n=4)	100	91-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.458	2.422	2.440	2.264
10	2.004	1.976	1.990	1.814
5	1.307	1.345	1.326	1.150
2.5	0.728	0.737	0.733	0.557
1.25	0.432	0.448	0.440	0.264
0.625	0.333	0.342	0.338	0.162
0.312	0.251	0.243	0.247	0.071
0	0.174	0.178	0.176

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Ischemic Preconditioning Alleviates Cerebral Ischemia–Reperfusion Injury by Interfering With Glycocalyx YN Zhang,Translational Stroke Research,2022
GLYCOCALYX SHEDDING DURING STEPWISE HEMODILUTION AND MICROVASCULAR PERMEABILITY B Ergin,University of Amsterdam,2020
Hemodilution causes glycocalyx shedding without affecting vascular endothelial barrier permeability in rats B Ergin,Research gate,2020
Resuscitation with pegylated-carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia P Guerci,Am J Physiol Renal Physiol,2020
Chemopreventive and hepatoprotective effects of Epigallocatechin-gallate against hepatocellular carcinoma: role of heparan sulfate proteoglycans pathway Mohamed M. Darweish et al,Journal of Pharmacy and Pharmacology,2014

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Function (From Uniprot)	Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Gene References into Functions	Plasma syndecan-1 and heparan sulfate correlate with microvascular glycocalyx degradation in hemorrhaged rats after different resuscitation fluids. PMID: 27037369 down-regulation of Syndecan-1 could inhibit VEGF-VEGFR-2 signaling through regulating internalization of VEGFR-2. PMID: 27075925 expression upregulated two months after renal transplantation PMID: 25972509 data suggest that after renal transplantation loss of hepatic HS together with increased syndecan-1 shedding hampers lipoprotein binding and uptake by the liver contributing to dyslipidemia PMID: 25154787 analysis of the molecular events that drive endocytosis of syndecan-1, a raft-dependent receptor and identification of its novel endocytic motif, MKKK PMID: 23525115 The abnormal expression of HSPG may be an important molecular mechanism that leads to the occurrence and development of proteinuria in adriamycin-induced nephropathy rats. PMID: 20506637 role in regulatory mechanism of apoptosis PMID: 11979781 Syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. PMID: 12135485 The major injury-related change, seen in injured brain and cultured glia, was an increase in 2-O-sulphated HS and increased syndecan-1, suggesting novel approaches to modulating scar formation. PMID: 18279312 Show More Hide All
Subcellular Location	Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
Protein Families	Syndecan proteoglycan family
Database Links	KEGG: rno:25216 STRING: 10116.ENSRNOP00000008582 UniGene: Rn.11176

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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