Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

Code CSB-E09813r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Alternative Names
Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
Abbreviation
SERPINA12
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %94
Range %90-98
1:4Average %91
Range %85-97
1:8Average %97
Range %92-104
Recovery
The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 105100-110
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.679 2.576 2.628 2.479
10002.071 2.114 2.093 1.944
5001.582 1.472 1.527 1.378
2500.958 0.913 0.936 0.787
1250.580 0.589 0.585 0.436
62.50.412 0.407 0.410 0.261
31.250.302 0.279 0.291 0.142
00.147 0.151 0.149
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin is a member of the serine protease inhibitor family which has insulin-sensitizing effects.The Rat Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin ELISA Kit is an essential tool for researchers in the metabolism and cardiovascular field. This quantitative assay is designed to detect and measure the level of Visceral Adipose Tissue-Derived Serine Protease Inhibitor (Vaspin) in serum, plasma, and tissue homogenates of Rattus norvegicus (Rat).

The assay principle of this kit is based on the sandwich method, which ensures accurate and reliable results. The detection range of the Rat Vaspin ELISA Kit is 31.25 pg/mL-2000 pg/mL, and the sensitivity is 7.8 pg/mL. The assay time for this kit is 1-5 hours, and the sample volume required is only 50-100 ul.

This kit's measurement is conducted at a detection wavelength of 450 nm, allowing for precise and efficient detection of Vaspin levels. Researchers can confidently use this kit to investigate the potential role of Vaspin in metabolism and cardiovascular diseases, given its specificity for visceral adipose tissue.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Expressed in visceral adipose tissues.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X