Rat alcohol dehydrogenase,ADH ELISA Kit

Code CSB-E11180r
Size 96T,5×96T,10×96T
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Product Details

Target Name
alcohol dehydrogenase,ADH
Alternative Names
Adh1 ELISA Kit; Adh-1 ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; EC 1.1.1.1 ELISA Kit; Alcohol dehydrogenase A subunit ELISA Kit
Abbreviation
ADH
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-95  
1:2 Average % 101  
Range % 96-105  
1:4 Average % 93  
Range % 88-97  
1:8 Average % 97  
Range % 91-103  
Recovery
The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-99  
EDTA plasma (n=4) 88 80-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.312 2.456 2.384 2.237  
10 1.586 1.712 1.649 1.502  
5 1.009 1.054 1.032 0.885  
2.5 0.641 0.632 0.637 0.490  
1.25 0.412 0.435 0.424 0.277  
0.625 0.287 0.274 0.281 0.134  
0.312 0.211 0.218 0.215 0.068  
0 0.146 0.147 0.147    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat ADH ELISA Kit was designed for the quantitative measurement of Rat ADH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Gene References into Functions
  1. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
  2. rat hepatic Class I ADH gene expression is regulated in vivo by ethanol in a mechanism involving the C/EBP site and the enhancer binding proteins beta and gamma PMID: 12213809
  3. Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization. PMID: 12631290
  4. activity and expression in gastrointestinal tissue homogenates PMID: 12787032
  5. class I alcohol dehydrogenase is induced by sterol regulatory element-binding protein-1 in rat liver PMID: 15123720
  6. Alcohol dehydrogenase PMID: 16109828
  7. Cellular expression of Adh1 in the GI tract was studied. PMID: 17257171
  8. A high ADH activity leads to low ethanol intake in animals. PMID: 17488809
  9. ADH class I gene is progesterone-responsive in the uterus. PMID: 18219182
  10. Results revealed that kidney alcohol dehydrogenase activities increased significantly after ethanol administration, but the electrophoretic pattern of alcohol dehydrogenase isoforms was unmodified. PMID: 16421892

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Subcellular Location
Cytoplasm.
Protein Families
Zinc-containing alcohol dehydrogenase family, Class-I subfamily
Database Links
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