Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-E10073r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 107 | 101-110 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Rat apolipoprotein H (Apo-H) ELISA Kit is the reliable and accurate solution for detecting and quantifying Beta-2-glycoprotein 1, also known as Apolipoprotein H (Apo-H) or Beta-2-glycoprotein I (B2GPI), in rat serum, plasma, and tissue homogenates.
With its detection range of 28 ng/mL-1800 ng/mL and a sensitivity of 7 ng/mL, this ELISA kit allows for precise measurements of Apo-H levels in a variety of sample types. The assay time is 1-5 hours, and the sample volume required is only 50-100 µL, making it a convenient and efficient choice for researchers in the cardiovascular field.
The Rat apolipoprotein H (Apo-H) ELISA Kit follows a sandwich assay principle, where the sample is captured by an immobilized antibody specific to rat Apo-H and then detected by a biotinylated antibody. The amount of detected signal is proportional to the concentration of Apo-H in the sample, which is measured at a detection wavelength of 450 nm. The high sensitivity, wide detection range, and easy-to-use protocol make this kit an ideal choice for researchers in the area.
There are currently no reviews for this product.
UniGene: Rn.1824
