Rat heat shock protein,HSP47 ELISA Kit

Product Details

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Target Background

Product Details

Target Name

serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

Alternative Names

Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit

Abbreviation

SERPINH1

Uniprot No.

P29457

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	92
	Range %	88-96
1:8	Average %	95
	Range %	91-99

Recovery

The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	87	83-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.311	2.507	2.409	2.261
5	1.740	1.645	1.693	1.545
2.5	1.087	1.124	1.106	0.958
1.25	0.664	0.652	0.658	0.510
0.625	0.420	0.433	0.427	0.279
0.312	0.339	0.327	0.333	0.185
0.156	0.226	0.230	0.228	0.080
0	0.147	0.148	0.148

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

The Rat HSP47/Serpin H1 ELISA Kit is a high-quality research tool designed for the quantitative detection of Serpin H1 in rat serum, plasma, tissue homogenates, and cell lysates. This kit is specifically designed for use in neuroscience research and can detect levels of Serpin H1 as low as 0.039 ng/mL with a detection range of 0.156 ng/mL to 10 ng/mL.

The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.

With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the HSP47 ELISA kit provides accurate and reliable results for your research needs. The kit includes all necessary components for the assay, such as microplate wells, wash buffer, detection antibody, and standards.

Target Background

Function
(From Uniprot)

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

Gene References into Functions

The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723

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Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

Serpin family

Database Links

KEGG: rno:29345

STRING: 10116.ENSRNOP00000022983

UniGene: Rn.98199

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Code	CSB-E11328r
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.