Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

Instructions
Code CSB-EL010868RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name heparan sulfate proteoglycan 2
Alternative Names RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly
Abbreviation HSPG2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %83
Range %80-86
1:400Average %97
Range %93-101
1:800Average %91
Range %85-97
1:1600Average %100
Range %93-107
Recovery
The recovery of rat HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)104100-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.766 2.798 2.782 2.680
5002.277 2.286 2.282 2.180
2501.593 1.562 1.578 1.476
1250.996 0.963 0.980 0.878
62.50.533 0.542 0.538 0.436
31.20.384 0.368 0.376 0.274
15.60.271 0.291 0.281 0.179
00.101 0.103 0.102
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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