Rat intestinal fatty acid binding protein,iFABP ELISA Kit

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Name: Rat intestinal fatty acid binding protein,iFABP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08026r

Product Details

Description

CUSABIO's IFABP rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IFABP in serum, plasma, urine, or tissue homogenates. This assay uses an antibody specific for rat IFABP coated on a 96-well plate. IFABP present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IFABP antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IFABP bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

FABP2 is the encoding gene for IFABP, which is mainly expressed in intestinal epithelial cells. IFABP is released into the circulation after small intestinal mucosal damage and its plasma levels have been linked to small intestinal diseases such as necrotizing enterocolitis and celiac disease.IFABP plays important roles in several steps of fat absorption and transport, including the uptake and trafficking of saturated and unsaturated long-chain fatty acids, targeting free fatty acids toward different metabolic pathways, protecting the cytosol from the cytotoxic effects of free fatty acids, and regulating the enzyme activity involved in lipid metabolism.

Target Name

fatty acid binding protein 2, intestinal

Alternative Names

Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit

Abbreviation

FABP2

Uniprot No.

P02693

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

1.25 ng/mL-80 ng/mL

Sensitivity

0.312 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-101
1:2	Average %	93
	Range %	87-99
1:4	Average %	90
	Range %	85-95
1:8	Average %	104
	Range %	98-108

Recovery

The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.695	2.671	2.683	2.536
40	1.915	1.901	1.908	1.761
20	1.306	1.334	1.320	1.173
10	0.884	0.899	0.892	0.745
5	0.517	0.537	0.527	0.380
2.5	0.375	0.384	0.380	0.233
1.25	0.211	0.233	0.222	0.075
0	0.145	0.149	0.147

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Gene References into Functions	Study characterized internal-water exchange and associated conformational dynamics in rIFABP by recording the water 2H and 17O MRD profiles for the apo and palmitate-bound forms PMID: 26012957 This work highlights the importance of the beta-barrel of IFABP for its specific functions. PMID: 25311169 the portal region of IFABP penetrates deeply into the phospholipid bilayer, especially when CL-containing vesicles are employed. The orientation of the protein and the degree of penetration were highly dependent on the lipid composition PMID: 24148314 n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARgamma pathway, and then upregulating tight junction protein expression. PMID: 22554584 Conjugated linoleic acid can improve insulin resistance of obese rat and increase the expression of fatty acid binding protein 2 mRNA. PMID: 16921753 Delta 78 Delta beta-barrel motif retains substantial beta-sheet content and preserves tertiary interactions, displaying cooperative unfolding and binding activity. Most strikingly, this construct adopts a remarkably stable dimeric structure in solution. PMID: 19844951 simulations of apo and holo-fatty acid binding protein: structure and dynamics of protein, ligand and internal water PMID: 11812142 Exchanging the alpha-helical domain of intestinal FABP (IFABP) for that of liver FABP drastically modifies the collisional mechanism characteristic of IFABP. PMID: 15035630 Increased concentrations of H-FABP significantly correlated with the concentrations of TNF- alpha in chronic heart failuare. PMID: 15277733 An examination of types of intermediates that are observed during the folding of this protein. PMID: 15723553 ionic interactions between IFABP and membranes appear to play a primary role in the process of fatty acid transfer to membranes, and hydrophobic interactions can also modulate the rates of ligand transfer PMID: 15863832 FABP2 gene polymorphism is associated in patients diagnosed with carotid artery atheroscelrosis. PMID: 16013194 analysis of intestinal fatty acid-binding protein stability and unfolding PMID: 16162507 there may be different collisional complex structures for holo- and apo-IFABP PMID: 16551626 The Gly121Val mutation of IFABP slows the formation of a normal nucleating site, not only slowing overall folding, but also allowing intermediates in regions distant from the mutation to be experimentally observed. PMID: 17615232 alphaI-helix is crucial for IFABP collisional FA transfer, and further indicate the participation of the alphaII-helix in the formation of a protein-membrane "collisional complex". PMID: 18284926 A structural explanation of the observed affinity of rat I-FABP for different drugs based on spectroscopic data and modeling experiments is presented.immunocytochemistry has been used to probe the expression of I-FABP in a cell culture model. PMID: 19160019 Delta98Delta (encompassing residues 29-126) emerges as a monomeric variant of intestinal fatty acid binding protein that folds properly, retaining functional activity, despite lacking extensive stretches involved in the closure of the beta-barrel. PMID: 19309727 Show More Hide All
Subcellular Location	Cytoplasm.
Protein Families	Calycin superfamily, Fatty-acid binding protein (FABP) family
Tissue Specificity	Expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips.
Database Links	KEGG: rno:25598 UniGene: Rn.91358

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Check Code：

Code	CSB-E08026r
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.