Rat intestinal fatty acid binding protein,iFABP ELISA Kit

Code CSB-E08026r
Size 96T,5×96T,10×96T
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Product Details

Target Name
fatty acid binding protein 2, intestinal
Alternative Names
Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
1.25 ng/mL-80 ng/mL
Sensitivity
0.312 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %89-101
1:2Average %93
Range %87-99
1:4Average %90
Range %85-95
1:8Average %104
Range %98-108
Recovery
The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)9389-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
802.695 2.671 2.683 2.536
401.915 1.901 1.908 1.761
201.306 1.334 1.320 1.173
100.884 0.899 0.892 0.745
50.517 0.537 0.527 0.380
2.50.375 0.384 0.380 0.233
1.250.211 0.233 0.222 0.075
00.145 0.149 0.147  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

CUSABIO's IFABP rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IFABP in serum, plasma, urine, or tissue homogenates. This assay uses an antibody specific for rat IFABP coated on a 96-well plate. IFABP present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IFABP antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IFABP bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

FABP2 is the encoding gene for IFABP, which is mainly expressed in intestinal epithelial cells. IFABP is released into the circulation after small intestinal mucosal damage and its plasma levels have been linked to small intestinal diseases such as necrotizing enterocolitis and celiac disease.IFABP plays important roles in several steps of fat absorption and transport, including the uptake and trafficking of saturated and unsaturated long-chain fatty acids, targeting free fatty acids toward different metabolic pathways, protecting the cytosol from the cytotoxic effects of free fatty acids, and regulating the enzyme activity involved in lipid metabolism.

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Target Background

Function
(From Uniprot)
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Gene References into Functions
  1. Study characterized internal-water exchange and associated conformational dynamics in rIFABP by recording the water 2H and 17O MRD profiles for the apo and palmitate-bound forms PMID: 26012957
  2. This work highlights the importance of the beta-barrel of IFABP for its specific functions. PMID: 25311169
  3. the portal region of IFABP penetrates deeply into the phospholipid bilayer, especially when CL-containing vesicles are employed. The orientation of the protein and the degree of penetration were highly dependent on the lipid composition PMID: 24148314
  4. n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARgamma pathway, and then upregulating tight junction protein expression. PMID: 22554584
  5. Conjugated linoleic acid can improve insulin resistance of obese rat and increase the expression of fatty acid binding protein 2 mRNA. PMID: 16921753
  6. Delta 78 Delta beta-barrel motif retains substantial beta-sheet content and preserves tertiary interactions, displaying cooperative unfolding and binding activity. Most strikingly, this construct adopts a remarkably stable dimeric structure in solution. PMID: 19844951
  7. simulations of apo and holo-fatty acid binding protein: structure and dynamics of protein, ligand and internal water PMID: 11812142
  8. Exchanging the alpha-helical domain of intestinal FABP (IFABP) for that of liver FABP drastically modifies the collisional mechanism characteristic of IFABP. PMID: 15035630
  9. Increased concentrations of H-FABP significantly correlated with the concentrations of TNF- alpha in chronic heart failuare. PMID: 15277733
  10. An examination of types of intermediates that are observed during the folding of this protein. PMID: 15723553
  11. ionic interactions between IFABP and membranes appear to play a primary role in the process of fatty acid transfer to membranes, and hydrophobic interactions can also modulate the rates of ligand transfer PMID: 15863832
  12. FABP2 gene polymorphism is associated in patients diagnosed with carotid artery atheroscelrosis. PMID: 16013194
  13. analysis of intestinal fatty acid-binding protein stability and unfolding PMID: 16162507
  14. there may be different collisional complex structures for holo- and apo-IFABP PMID: 16551626
  15. The Gly121Val mutation of IFABP slows the formation of a normal nucleating site, not only slowing overall folding, but also allowing intermediates in regions distant from the mutation to be experimentally observed. PMID: 17615232
  16. alphaI-helix is crucial for IFABP collisional FA transfer, and further indicate the participation of the alphaII-helix in the formation of a protein-membrane "collisional complex". PMID: 18284926
  17. A structural explanation of the observed affinity of rat I-FABP for different drugs based on spectroscopic data and modeling experiments is presented.immunocytochemistry has been used to probe the expression of I-FABP in a cell culture model. PMID: 19160019
  18. Delta98Delta (encompassing residues 29-126) emerges as a monomeric variant of intestinal fatty acid binding protein that folds properly, retaining functional activity, despite lacking extensive stretches involved in the closure of the beta-barrel. PMID: 19309727

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Subcellular Location
Cytoplasm.
Protein Families
Calycin superfamily, Fatty-acid binding protein (FABP) family
Tissue Specificity
Expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips.
Database Links

KEGG: rno:25598

UniGene: Rn.91358

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