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CUSABIO's IFABP rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IFABP in serum, plasma, urine, or tissue homogenates. This assay uses an antibody specific for rat IFABP coated on a 96-well plate. IFABP present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IFABP antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IFABP bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
FABP2 is the encoding gene for IFABP, which is mainly expressed in intestinal epithelial cells. IFABP is released into the circulation after small intestinal mucosal damage and its plasma levels have been linked to small intestinal diseases such as necrotizing enterocolitis and celiac disease.IFABP plays important roles in several steps of fat absorption and transport, including the uptake and trafficking of saturated and unsaturated long-chain fatty acids, targeting free fatty acids toward different metabolic pathways, protecting the cytosol from the cytotoxic effects of free fatty acids, and regulating the enzyme activity involved in lipid metabolism.
|Target Name||fatty acid binding protein 2, intestinal|
Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
|Species||Rattus norvegicus (Rat)|
|Sample Types||serum, plasma, urine, tissue homogenates|
|Detection Range||1.25 ng/mL-80 ng/mL|
|Detection Wavelength||450 nm|
|Research Area||Signal Transduction
|ELISA Data Analysis||ELISA Standard Curve & Curve Expert software|
|ELISA kit FAQs|
|Storage||Store at 2-8°C. Please refer to protocol.|
|Lead Time||3-5 working days|
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FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
|Gene References into Functions||
|Protein Families||Calycin superfamily, Fatty-acid binding protein (FABP) family|
|Tissue Specificity||Expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips.|