Rat monoamine oxidase(MAO) ELISA Kit

Code CSB-E15076r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
monoamine oxidase(MAO)
Alternative Names
Maoa; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
Abbreviation
MAO
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
93.75 mU/mL-6000 mU/mL
Sensitivity
23.43 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 92  
Range % 88-96  
1:10 Average % 96  
Range % 90-102  
1:20 Average % 102  
Range % 98-104  
1:40 Average % 87  
Range % 83-92  
Recovery
The recovery of rat MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 85-99  
EDTA plasma (n=4) 89 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
6000 2.633 2.866 2.750 2.557  
3000 1.787 1.881 1.834 1.641  
1500 1.123 1.188 1.156 0.963  
750 0.689 0.705 0.697 0.504  
375 0.502 0.525 0.514 0.321  
187.5 0.369 0.360 0.365 0.172  
93.75 0.245 0.236 0.241 0.048  
0 0.195 0.191 0.193    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat MAO ELISA Kit was designed for the quantitative measurement of Rat MAO protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 mU/mL-6000 mU/mL and the sensitivity is 23.43 mU/mL.

Customer Reviews and Q&A

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 Q&A
Q:

May I know the composition of stop solution that is used for the monoamine oxidase ELISA Kit?

A:
Thanks for your inquiry. The stop solution of this kit is 4N sulfuric acid.Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
Subcellular Location
Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Protein Families
Flavin monoamine oxidase family
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