Rat myelin basic protein,MBP ELISA Kit

Code CSB-E08284r
Size 96T,5×96T,10×96T
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Product Details

Target Name
myelin basic protein
Alternative Names
MbpMyelin basic protein ELISA Kit; MBP ELISA Kit
Abbreviation
MBP
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-93
1:2Average %99
Range %95-103
1:4Average %92
Range %88-96
1:8Average %95
Range %91-100
Recovery
The recovery of rat MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)9592-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.333 2.173 2.253 2.086
101.402 1.377 1.390 1.223
50.868 0.888 0.878 0.711
2.50.598 0.583 0.591 0.424
1.250.425 0.412 0.419 0.252
0.6250.298 0.312 0.305 0.138
0.3120.260 0.251 0.256 0.089
00.168 0.166 0.167  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat MBP ELISA Kit was designed for the quantitative measurement of Rat MBP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Target Background

Function
(From Uniprot)
Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Gene References into Functions
  1. Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID: 29742816
  2. A role for the retrograde dynein/dynactin motor complex in anterograde mbp mRNA transport. PMID: 29073112
  3. The aberrant phase transition of MBP molecules from their cohesive to soluble and non-adhesive state is a mechanism triggering myelin breakdown in neuromyelitis optica. PMID: 27346352
  4. hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression PMID: 27230884
  5. Bilateral common carotid artery occlusion led to loss of myelin basic protein expression. PMID: 26670084
  6. Study demonstrated that downregulation of tau severely impairs process outgrowth, oligodendrocyte differentiation, and sheet formation, and that MBP mRNA transport to the distal cellular extensions is disturbed PMID: 25847153
  7. Protein levels of MBP were significantly greater in orbital frontal cortex (OFC) but not dorsal striatum (dSTR) of females as compared to males, suggesting that myelination is increased in OFC but similar in dSTR of females as compared to males PMID: 26002313
  8. MBP cell membrane localization is altered in the presence of galactolipids. PMID: 25003183
  9. Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4. PMID: 25512606
  10. The expression of myelin basic protein was detected in accordance with the radially extending myelinated processes of the neurons in the cortex. PMID: 25282817
  11. Given the principle of the longest time window and the lowest therapeutic dose, the optimized therapeutic dose and time window should be injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia in cerebral ischemic injury. PMID: 24524292
  12. The e-MBP isoform is exclusively present in OLN-93 cells. PMID: 24321769
  13. MBP epitopes normally sheltered from immunosurveillance may induce MBP-specific T cell clones and a self-sustaining immune reaction, which may together contribute to transition of acute pain into chronic neuropathic pain state. PMID: 22676642
  14. Methylmercury-induced perturbation in Mbp gene expression occurred as a consequence of decreased oligodendrocyte cell population in absence of a significant impact on its post-transcriptional regulation. PMID: 22835759
  15. Morphological basis of spinal cord recovery from debilitating experimental neurodenerative disease is evidenced by robust myelin basic protein (MBP) expression and extensive remyelination. PMID: 21996274
  16. The impact of Hoxa2 over-expression on the proliferation and differentiation of CG4-SHoxa2 cells was investigated. PMID: 21479584
  17. MBP synthesis is regulated by hnRNP-K and integrin activation. PMID: 21357748
  18. Myelin basic protein expression decreases following demyelination of optic nerve and chiasm and then increases to control levels 14 days post-lesion. PMID: 20637745
  19. Sp1 phosphorylation can be regulated by PKC-ERK pathways. This phosphorylation is important for MBP transcription and oligodendrocyte differentiation PMID: 20882567
  20. Increasing the negative surface charge of the cell membrane reduces the amount of Fyn-SH3 domain binding to membrane-associated MBP. PMID: 19178193
  21. Phosphatidylinositol-3 kinase p85 enhances expression from the myelin basic protein promoter in oligodendrocytes independent of PI3K activity and dependent on the adapter functions of the p85 subunit's SH2 domains PMID: 12390518
  22. isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform. The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. PMID: 14580679
  23. Two regulatory elements in the promoter interact with Nkx2.2 in vitro PMID: 15695521
  24. Susceptibility or resistance to EAE is not regulated by the amount of the neuroantigens expressed in the thymus. Furthermore, the lesion distribution, but not the clinical course, of EAE is related to the neuroantigen expression in the CNS. PMID: 16773652
  25. Mbp is a binding partner and calmodulin adaptor for the BKCa channel. PMID: 17610306
  26. 21.5 kDa molecule expressed during [dental] hard tissue formation PMID: 18821983
  27. study demonstrates co-localisation of MBP & myristoylated alanine-rich C kinase substrate (MARKS) in oligodendrocytes, & co-distribution of MBP, MARCKS & GAP-43 in lipid raft fractions recovered from myelin & brain homogenates PMID: 19026719
  28. Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis. PMID: 19211156

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Subcellular Location
Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
Protein Families
Myelin basic protein family
Tissue Specificity
Found in both the central and the peripheral nervous system.
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