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Rat neurotensin (NTS) ELISA kit

Protocol
Code CSB-EL016136RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
neurotensin
Alternative Names
NtsNeurotensin/neuromedin N [Cleaved into: Large neuromedin N ELISA Kit; NmN-125); Neuromedin N ELISA Kit; NN ELISA Kit; NmN); Neurotensin ELISA Kit; NT); Tail peptide] ELISA Kit
Abbreviation
NTS
Uniprot No.
P20068
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 92
Range % 88-96
1:2 Average % 84
Range % 80-88
1:4 Average % 95
Range % 90-99
1:8 Average % 89
Range % 85-93
Recovery
The recovery of rat NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-103
EDTA plasma (n=4) 100 95-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.541 2.379 2.460 2.253
500 1.839 1.721 1.780 1.573
250 1.152 1.205 1.179 0.972
125 0.785 0.816 0.801 0.594
62.5 0.503 0.482 0.493 0.286
31.2 0.348 0.328 0.338 0.131
15.6 0.294 0.303 0.299 0.092
0 0.203 0.210 0.207 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat NTS ELISA Kit was designed for the quantitative measurement of Rat NTS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Nts Proteins

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Target Background

Function
(From Uniprot)
Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.
Subcellular Location
Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Packaged within secretory vesicles.
Protein Families
Neurotensin family
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