Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

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Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Name: Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Brand: CUSABIO
SKU: CSB-E08491r

Citations (11) Protocol MSDS

Code	CSB-E08491r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (11)
Customer Reviews and Q&A

Target Name

tartrate-resistant acid phosphatase 5b,TRACP-5b

Abbreviation

TRACP-5b

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 mIU/mL-20 mIU/mL

Sensitivity

0.078 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	100
	Range %	97-103
1:4	Average %	93
	Range %	88-97
1:8	Average %	98
	Range %	93-102

Recovery

The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	95	91-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
20	2.076	1.988	2.032	1.887
10	1.292	1.196	1.244	1.099
5	0.804	0.820	0.812	0.667
2.5	0.511	0.521	0.516	0.371
1.25	0.324	0.335	0.330	0.185
0.625	0.282	0.278	0.280	0.135
0.312	0.223	0.212	0.218	0.073
0	0.146	0.144	0.145

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Tartrate-resistant acid phosphatase 5b (TRACP-5b) is a lysosomal enzyme that plays an important role in bone metabolism and remodeling. Osteoclasts primarily secrete this enzyme during bone resorption. It serves as a specific biomarker for osteoclast activity. TRACP-5b levels in circulation reflect the rate of bone resorption, making it a valuable indicator for studying bone turnover dynamics, osteoporosis research, and metabolic bone diseases. Its resistance to tartrate inhibition distinguishes it from other acid phosphatase isoforms, providing specificity for bone-related research applications.

The Rat tartrate-resistant acid phosphatase 5b ELISA Kit (CSB-E08491r) is designed for quantitative measurement of TRACP-5b in rat samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 0.312-20 mIU/mL and sensitivity of 0.078 mIU/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, targeting Rattus norvegicus TRACP-5b for research applications.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in bone metabolism research to quantify tartrate-resistant acid phosphatase 5b as a bone resorption marker in rat serum samples. Researchers have applied the assay alongside other bone biomarkers to comprehensively evaluate skeletal metabolism in experimental studies.

• Bone resorption measurement: Used to measure TRACP-5b levels in serum as an indicator of osteoclast activity and bone breakdown processes
• Bone metabolism profiling: Applied in conjunction with bone formation markers like osteocalcin to evaluate overall skeletal turnover
• Biochemical analysis: Incorporated into comprehensive serum biochemical panels for bone-related research studies in rat models

Unveiling the Potential Effect of Nattokinase Against Radiation-Induced Acute Lung Injury in Rats NM Elbakary, RE Maarouf, AA Hassan,Natural Product Communications,2025
An aqueous extract of Prunella vulgaris L. ameliorates cadmium-induced bone loss by promoting osteogenic differentiation in female rats L Mo,Food and chemical toxicology,2023
Radiotherapy-induced bone deterioration is exacerbated in diabetic rats treated with streptozotocin M Jiang,The Brazilian Journal of Medical and Biological Research,2021
Tianchang Capsule prevents ovariectomy induced osteoporosis in rats H CHEN,Food Science and Technology,2021
Irisin Has a Protective Role against Osteoporosis in Ovariectomized Rats EN Morgan,BioMed Research International,2021
Effect of Qing'e Decoction on Leptin/Leptin Receptor and Bone Metabolism in Naturally Aging Rats P Sun,Evidence-Based Complementary and Alternative Medicine,2020
Differential skeletal response in adult and aged rats to independent and combinatorial stimulation with pulsed electromagnetic fields and mechanical vibration Cai J,FASEB J,2020
Boldine isolated from Litsea cubeba inhibits bone resorption by suppressing the osteoclast differentiation in collagen-induced arthritis Zhao H.et al,Int Immunopharmacol.,2017
Effects of rabeprazole on bone metabolic disorders in a gastrectomized rat model. Yamasaki Y.et al,Biomed Rep.,2016
Advanced Oxidation Protein Products Accelerate Bone Deterioration in Aged Rats Zeng JH et al,Exp Gerontol,2013
A role for suppressed bone formation favoring catch-up fat in the pathophysiology of catch-up growth after food restriction Xiangfei Guo et al,European Journal of Nutrition,2011

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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