Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit

Code CSB-E17373r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
tumor necrosis factor-inducible gene 6 protein(TSG-6)
Alternative Names
TSG-6, TSG6, hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor-inducible protein 6|tumor necrosis factor-stimulated gene-6 protein
Abbreviation
TSG-6
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TSG-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 87-95  
1:2 Average % 101  
Range % 97-104  
1:4 Average % 91  
Range % 87-94  
1:8 Average % 100  
Range % 96-104  
Recovery
The recovery of rat TSG-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 91-98  
EDTA plasma (n=4) 94 91-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.545 2.532 2.539 2.373  
10 1.903 1.867 1.885 1.719  
5 1.210 1.222 1.216 1.050  
2.5 0.683 0.718 0.701 0.535  
1.25 0.429 0.448 0.439 0.273  
0.625 0.341 0.332 0.337 0.171  
0.312 0.256 0.243 0.250 0.084  
0 0.168 0.164 0.166    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TSG-6 ELISA Kit was designed for the quantitative measurement of Rat TSG-6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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