Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Code CSB-E11298r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
beta-2-microglobulin
Alternative Names
B2MBeta-2-microglobulin ELISA kit
Abbreviation
B2M
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
0.156 μg/mL-10 μg/mL
Sensitivity
0.039 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %96
Range %92-101
1:4Average %100
Range %95-105
1:8Average %90
Range %85-95
Recovery
The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
102.645 2.678 2.662 2.515
51.855 1.896 1.876 1.729
2.51.256 1.263 1.260 1.113
1.250.834 0.869 0.852 0.705
0.6250.487 0.489 0.488 0.341
0.3120.325 0.353 0.339 0.192
0.1560.261 0.284 0.273 0.126
00.145 0.149 0.147  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is your go-to solution for accurate and reliable quantification of Beta-2-microglobulin in rat serum, plasma, urine, and tissue homogenates.

Designed for researchers in the field of immunology、autoimmune diseases and renal function research, this ELISA kit provides a quantitative measurement of B2M levels with a detection range of 0.156 μg/mL-10 μg/mL and a sensitivity of 0.039 μg/mL, ensuring precise and reproducible results.

The assay principle is based on a sandwich measurement, where the captured rat β2-microglobulin is detected using a specific antibody conjugated to horseradish peroxidase. The kit's assay time is 1-5 hours, and the sample volume required is between 50-100ul.

The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is compatible with standard 96-well microplates and requires a detection wavelength of 450 nm. This ELISA kit has been cited in more than 3 scientific publications, demonstrating its robust performance and reliability.

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Target Background

Function
(From Uniprot)
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Subcellular Location
Secreted.
Protein Families
Beta-2-microglobulin family
Database Links
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