Human corticotropin releasing hormone,CRH ELISA Kit

Instructions
Code CSB-E06872h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name corticotropin releasing hormone
Alternative Names CRHCorticoliberin ELISA Kit; Corticotropin-releasing factor ELISA Kit; CRF ELISA Kit; Corticotropin-releasing hormone ELISA Kit
Abbreviation CRH
Uniprot No. P06850
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1 ng/mL-40 ng/mL
Sensitivity 1 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-103  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 95  
Range % 88-98  
1:8 Average % 95  
Range % 90-101  
Recovery
The recovery of human CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 95-102  
EDTA plasma (n=4) 96 91-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
0 1.911 1.838 1.875    
1 1.308 1.297 1.303    
3 1.025 1.005 1.015    
9.2 0.751 0.736 0.744    
20 0.570 0.543 0.557    
40 0.351 0.342 0.347    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).
Gene References into Functions
  1. Early-preterm birth showed a syndrome of high maternal CRH and low vitamin D status. PMID: 28780891
  2. REVIEW: CRF Family of Neuropeptides and their Receptors - Mediators of the Central Stress Response PMID: 28260504
  3. REVIEW: Brain and Gut CRF Signaling: Biological Actions and Role in the Gastrointestinal Tract PMID: 28240194
  4. REVIEW: The Multi-faceted Profile of Corticotropin-releasing Factor (CRF) Family of Neuropeptides and of their Receptors on the Paracrine/Local Regulation of the Inflammatory Response PMID: 28103784
  5. Significant effects of CNS sensitization on CRF mRNA levels were detected in the Dorsal Striatum (increasing effect). PMID: 29857328
  6. These results show epigenetic changes in the CRH gene related to severity of suicide attempt in adults and a general psychiatric risk score in adolescents. PMID: 29277323
  7. Data suggest that corticotrophin-releasing hormone (CRH) is able to stimulate copeptin release in healthy controls suggesting direct interaction of CRH/CRH-receptor signaling and vasopressin; these interactions appear to be altered in patients with pituitary disease; copeptin may be serum biomarker of altered CRH/CRH-receptor signaling in pituitary diseases. PMID: 28795329
  8. The development of disorders related to heightened stress sensitivity and dysregulation of stress-coping mechanisms appears to involve regulatory mechanisms of the CRH family members. The purpose of this review is to summarize the most significant discoveries related to CRH over time. [review] PMID: 28071586
  9. A significant increase in CRH and CRHR-1 expression was significantly correlated with psychological stress in vitiligo. PMID: 27663146
  10. Corticotrophin-releasing hormone accelerated tumor angiogenesis by upregulating VEGF expression and secretion in colon cancer cells. PMID: 28618089
  11. This study found that the expressions of CRH and CRHR1 were significantly higher in the epileptogenic tissues of patients with IS than in the control group. PMID: 27534449
  12. The authors propose that conditions impacting on epiallele distribution influence the number of transcriptionally active CRH gene copies in the trophoblast cell population determining the gestational trajectory of placental CRH production in normal and pathological pregnancies. PMID: 28151936
  13. Neuroimmune-endocrine events may lead to overactivity of sympathetic nervous system that triggers cascade of pathologic conditions in ovary in polycystic ovary syndrome (PCOS). Data suggest that women with PCOS exhibit reduction of CRH and NGF; reduction of CRH and NGF may be under influence of sympathetic nervous system and may reflect deficit of neuronal stress-adaptation in PCOS patients. (NGF = nerve growth factor) PMID: 27908212
  14. brain activation in response to colorectal distention is enhanced after CRH injection in Irritable bowel syndrome patients compared to healthy controls PMID: 27448273
  15. In deep infiltrating endometriotic lesions, CRH, Ucn and CRH-R2 mRNA levels were significantly higher than in ovarian endometrioma. PMID: 27567427
  16. In summary, cardiac expression of CRFR1, CRF, and Ucn3 genes is elevated in heart failure and may contribute to the activation of the CRF/Ucn system in these patients. PMID: 27754786
  17. VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts. PMID: 26818776
  18. This study localized a complete CRF system in the human fetal heart. PMID: 27377597
  19. Placenta CRH mRNA concentration appears to convey information about the risk of brain damage in the infant born at an extremely low gestational age. PMID: 26331704
  20. These results suggest that the CRH and CRH-BP genes have no direct effect on Irritable bowel syndrome status. PMID: 26882083
  21. This study evaluated the associations of CRH polymorphisms with susceptibility to MDD and response to antidepressant treatment PMID: 26055202
  22. results suggest that glucocorticoids induce a transcription complex consisting of RelB/p52, CBP, and HDAC1 that triggers a dynamic acetylation-mediated epigenetic change to induce CRH expression in full-term human placenta. PMID: 26307012
  23. Interaction of RelB with the CRH or COX-2 gene promoters decreased when STAT3 was depleted. PMID: 25771405
  24. the present study demonstrates that the expression of CRH/CRH-R1 in lesions of chronic plaque psoriasis is lower than that in psoriatic perilesional skin and normal control skin. PMID: 25630718
  25. results show that activation of CRH receptors by CRH ligands stimulates VEGF-A expression in intestinal epithelial cells through the cAMP/CREB pathway PMID: 26350463
  26. This corticotropin releasing factor-induced regulation on norepinephrine transporter expression and function may play a role in development of stress-related depression and anxiety PMID: 26212818
  27. Placental CRH exposure may make a unique contribution to fetal programming of obesity risk. PMID: 25591114
  28. Data suggest that signal transduction involving corticotropin-releasing factor (CRH) and CRH receptors (CRHR) taking place in cochlea are involved in protecting against noise-induced hearing loss. [REVIEW] PMID: 26074267
  29. Production of CRF and UCN1 in human dendritic cells is strongly augmented by commensal intestinal bacteria. PMID: 25339828
  30. we show that Abeta species can hyperexcite CRF neurons, providing a mechanism by which Abeta influences stress-related symptoms PMID: 25673853
  31. High serum corticotropin-releasing hormone (CRH) and bone marrow mast cell CRH receptor expression in a mastocytosis patient. PMID: 24985398
  32. Corticotrophin-Releasing Factor (CRF) and the urocortins are potent regulators of the inflammatory phenotype of human and mouse white adipocytes. PMID: 24835211
  33. Results suggest that ULBP2 is expressed and released from cervical cancer cells by CRF, which regulates NKG2D expression in natural killer cells. PMID: 24841552
  34. This review focuses on the CRF receptor, a stress-related peptide implicated in diverse psychiatric and medical disorders that are more prevalent in females in diverse psychiatric and medical disorders that are more prevalent in females. PMID: 23849813
  35. evidence suggests that CRH regulates neurogenesis in the brain. PMID: 23380766
  36. as labor progresses, progesterone and corticotropin-releasing hormone increase and subsequently decrease precipitously in the immediate postpartal period PMID: 24597258
  37. Data suggest that expression of CRF, CRF-related neuropeptides, and CRF receptors in the liver plays crucial regulatory roles in homeostasis, in allostasis to endogenous stresses, and in adaptation to exogenous stress stimuli. [REVIEW] PMID: 23933692
  38. During the midluteal phase of the cycle, metformin may decrease the production of CRH and urocortin in the endometrium. PMID: 23987517
  39. in human melanoma HMV-II cells both CRF and Ucn1 regulate TRP1 gene expression via Nurr-1/Nur77 production, independent of pro-opiomelanocortin or alpha-melanocyte-stimulating hormone stimulation. PMID: 23416839
  40. IL-6 and CRH are both secreted in a pulsatile fashion during the active phase of human labor. The time-integrated concentrations of the two hormones are positively correlated, with IL-6 leading CRH secretion. PMID: 23928667
  41. ERK1/2 activation in response to CRH is biphasic, involving a first cAMP- and B-Raf-dependent early phase and a second phase that critically depends on CRHR1 internalization and beta-arrestin2. PMID: 23371389
  42. corticotrophin-releasing hormone (CRH) might play a significant role in the pathogenesis of intrahepatic cholestasis of pregnancy(ICP) and provide a new approach to further investigate the etiology of ICP PMID: 23478074
  43. A novel CRH missense mutation (Pro30Arg) results in reduced levels of protein secretion in the short time thus suggesting that mutated people could present an altered capability to respond immediately to stress agents. PMID: 23593457
  44. CRH/CRHR1 are involved in regulation of PGHS2 (prostaglandin-endoperoxide synthase 2) expression in myometrium before/during labor; these actions appear important in priming myometrium for labor and adaptive responses to inflammatory mediators. PMID: 23666959
  45. Data suggest that CRF plays role in acute postinjury gut dysfunction; CRF is expressed in small bowel of elective/emergency gastrointestinal (GI) surgery patients with concomitant GI dysfunction; plasma CRF correlates with poor gastric emptying. PMID: 23466050
  46. Postnatally stressed adult transgenic mice have decreased hippocampal nectin-3 levels, which could be attenuated by CRHR1 inactivation and mimicked by corticotropin-releasing hormone (CRH) overexpression in forebrain neurons. PMID: 23644483
  47. the age-associated changes in the expression of CRH system components reflect an exaggerated stress response reaction, putting the aged skin continuously in a stress-like situation. PMID: 22533365
  48. CRH may be involved in the pathogenesis of gastric cancer PMID: 23477585
  49. study demonstrates that the HeLa cervical cancer cell line expresses CRH; additionally, CRH was shown to induce FasL expression PMID: 23076876
  50. The aberrant expression of corticotropin-releasing hormone in pre-eclampsia may activate the FasL-positive decidual macrophages, impair the physiological turnover of extravillous trophoblasts, and eventually disturb placentation. PMID: 22763913
  51. CRH acts on CRHR1 to activate different signaling pathways before and after onset of labor, thereby resulting in differential calcium signaling in response to CRH. PMID: 22869609
  52. RelB/NF-kappaB2, is constitutively activated in the human placenta, which binds to a previously undescribed NF-kappaB enhancer of corticotropin-releasing hormone (CRH) gene promoter to regulate CRH expression. PMID: 22734038
  53. These data demonstrate that CRF triggers increases in intestinal paracellular permeability via mast cell dependent release of TNF-alpha and proteases. PMID: 22768175
  54. no association found between promoter polymorphisms and preterm birth in a Hispanic population PMID: 22608025
  55. Stress-induced response of CRH is differentially modulated by CRH promoter polymorphisms in rheumatoid arthritis patients. PMID: 22703673
  56. The results give evidence of a possible participation of CRF and receptor for endothelin B in the pathogenesis of the itch in the dermatitis herpetiformis and bullous pemphigoid. PMID: 22535604
  57. increased serum levels in psoriasis and atopic dermatitis patients PMID: 22360979
  58. The results of this study suggested genetic increases in the brain of CRF expression mimicked chronic stress on the effects of amyloid deposition, neurodegeneration, and behavioral deficits. PMID: 22045495
  59. review tissue specific differences in CRH gene expression, and discuss how epigenetic chromatin modification mechanisms can relate to tissue specific differences in expression of CRH (review) PMID: 22201770
  60. in placenta, CRH produced locally regulates GLUT1 and GLUT3 expression, CRHR1 and CRHR2-mediated differential regulation of GLUT1 and GLUT3 expression PMID: 22234467
  61. study suggests that in humans and in rats, septic shock is associated with decreased ACTH synthesis that is not compensated by its two natural secretagogues, AVP and CRH PMID: 22073145
  62. Confirm the anti-inflammatory function of VIP, through the modulation of the expression of CRF system that impacts in a reduction of mediators with inflammatory/destructive functions. PMID: 21360527
  63. The immunomodulatory effects of CRH may include an important, albeit not explored yet, role in epidermal tissue remodeling and regeneration and maintenance of tissue homeostasis. PMID: 21765902
  64. REVIEW: role of corticotropin-releasing hormone in the pathophysiology of depression PMID: 21261589
  65. central AT(1) receptor signaling plays a homeostatic role in the regulation of food intake by maintaining gene expression of Crh in hypothalamus PMID: 21525005
  66. The corticotropin-releasing factor (CRF) system is principal neuroendocrine coordinator of stress responses involved in inflammatory process in the gastrointestinal tract via vagal and peripheral pathways, as implied by multiple reports reviewed here. PMID: 19379831
  67. Women with endometriosis show an impaired endometrial expression of CRH and Ucn mRNA. PMID: 21289256
  68. A large literature dealing with various stressors that regulate gonadotrophin-releasing hormone secretion in a variety of species provides evidence that stress modulates GnRH secretion by activating the corticotrophin-releasing factor system--REVIEW PMID: 20727865
  69. Our findings showed a significant impact of premature rupture of membranes with chorioamnionitis on placental CRH peptides and receptors, suggesting that placental expression of stress-related pathways is activated in infective process PMID: 21106714
  70. Higher midpregnancy placental CRH was not associated with an increased risk of postpartum depression. PMID: 20943787
  71. The phosphorylation of CREB contributes to the activation of CRF gene transcription after the deprivation of glucocorticoids in hypothalamic cells. PMID: 20626567
  72. Forskolin-stimulated corticotropin releasing factor gene transcription is mediated by CRE, which included from - 220 to - 233, on the CRF 5'-promoter region. PMID: 20472145
  73. The elevated level of placental CRH may be a trigger of the transcription of its own receptor CRH-R1 during caesarian operations. PMID: 17294739
  74. We demonstrate that human skin is an extramedullary reservoir for mast cell precursors, and we have identified a regulatory loop between CRH and stem cell factor signaling PMID: 20043013
  75. Validated animal models of the stress component of addiction suggest that corticotropin-releasing factor systems are a viable target for the development of future medications for drug addiction--REVIEW PMID: 20047160
  76. Findings of slightly diminished cerebrospinal fluid CRH levels after feeding may be accounted for by the rises in glucocorticoids and their associated negative feedback effects on central nervous system CRH. PMID: 11406119
  77. during pregnancy CRH primarily activates intracellular signals that contribute to the maintenance of myometrial quiescence. PMID: 11854458
  78. NPY regulates the CRH system of the human hypothalamus and some of the effects of NPY on metabolic, autonomic, and endocrine functions may be mediated through CRH PMID: 11932939
  79. The ability of CRH to induce POMC expression in cultured melanoma cells; in CRH positive melanomas, seven out of nine cases (78%) of primary melanoma, and 7 out of 12 cases (58%) of MetM showed colocalization of CRH and POMC peptides PMID: 11936276
  80. AP-2alpha modulates human corticotropin-releasing hormone gene expression in the placenta by direct protein-protein interaction. PMID: 12062896
  81. CRH modulates human cardiovascular and pupillary autonomic reflexes. PMID: 12114295
  82. Intraventricular insulin potentiates the anorexic effect of corticotropin releasing hormone in rats PMID: 12429558
  83. CRH exerts inhibitory effects on steroidogenesis in rat and human granulosa cells, acting through the CRH receptor. PMID: 12450317
  84. Placenta takes part in stress syndrome by releasing CRF, which may help to influence uterine perfusion, maternal metabolism, fluid balance, and uterine contractility, protecting fetus from hostile environment.(review) PMID: 12583606
  85. in chorion trophoblast cells, endogenously produced corticotropin-releasing hormone exerts a tonic stimulatory effect on 15-hydroxyprostaglandin dehydrogenase activity PMID: 12679466
  86. Detected in placenta, myometrium, decidua, and fetal membranes. Widespread expression of CRH system in gestational tissue suggests paracrine role CRH in birth process (e.g. effects on macrophages and endothelial cells). PMID: 12784189
  87. reduced levels of circulating corticotropin releasing factor were associated with increased uterine artery resistance PMID: 12853091
  88. human cord blood-derived cultured mast cells (hCBMC) at 10 wk, but not at 2 wk, are immunocytochemically positive for CRH and urocortin PMID: 14576187
  89. progesterone, corticotropin-releasing factor, and activin A have roles in paracrine regulation of endometrial function [review] PMID: 14667971
  90. corticotropin-releasing hormone inhibits nuclear factor-kappaB binding and transcriptional activity. PMID: 14675201
  91. An association between an allele of the CRH-linked locus and behavioral inhibtion. Among offspring of parents with panic disorder, this association was marked. PMID: 14675801
  92. CRH administration induced sympathotonic effects which were antagonized by ANP PMID: 14698679
  93. Expression of urocortin mRNA is significantly downregulated in the Edinger-Westphal nucleus of CRF-overexpressing transgenic mice compared with wild-type. PMID: 14706771
  94. The high expression of CRH in placenta and in choriodecidua and myometrium suggested that CRH derived from placenta exerts direct or indirect actions on these tissues. PMID: 15072543
  95. CRH promoter region between -248 and -213 bp was essential for the effect of both E2 and ICI 182780. This region contains a consensus cAMP regulatory element that is a requirement for E2- and ICI 182780-mediated activity. PMID: 15126558
  96. CRH inhibited the transition of keratinocytes from G0/1 to S phase of the cell cycle, which was accompanied by an increased expression of cdk inhibitor p16 (Ink4a) protein PMID: 15468147
  97. calcium/calmodulin-dependent protein kinase IV signaling pathway may play an important role in the excitation-mediated regulation of CRH synthesis PMID: 15591024
  98. CRH regulates IL-18 production through the MAPK signaling pathway in human keratinocytes. PMID: 15816833
  99. Ileal-specific CRF promotes inflammation and modulates bowel motility. PMID: 15883387
  100. CRH genetic alterations could explain several autosomal dominant nocturnal frontal lobe epilepsy clinical features PMID: 16222669

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Subcellular Location Secreted
Protein Families Sauvagine/corticotropin-releasing factor/urotensin I family
Tissue Specificity Produced by the hypothalamus and placenta.
Database Links

HGNC: 2355

OMIM: 122560

KEGG: hsa:1392

STRING: 9606.ENSP00000276571

UniGene: Hs.75294

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