Human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit

Code CSB-E11790h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name dihydropyrimidinase-like 3
Alternative Names Collapsin response mediator protein 4 ELISA Kit; Collapsin response mediator protein 4 long ELISA Kit; CRMP 4 ELISA Kit; CRMP-4 ELISA Kit; CRMP4 ELISA Kit; Dihydropyrimidinase like 3 ELISA Kit; Dihydropyrimidinase related protein 3 ELISA Kit; Dihydropyrimidinase-related protein 3 ELISA Kit; DPYL3_HUMAN ELISA Kit; DPYSL3 ELISA Kit; DRP 3 ELISA Kit; DRP-3 ELISA Kit; DRP3 ELISA Kit; LCRMP ELISA Kit; TUC4 ELISA Kit; ULIP 1 ELISA Kit; ULIP ELISA Kit; ULIP-1 ELISA Kit; ULIP1 ELISA Kit; UNc 33 like phosphoprotein 1 ELISA Kit; Unc 33 like phosphoprotein ELISA Kit; Unc-33-like phosphoprotein 1 ELISA Kit
Abbreviation DPYSL3
Uniprot No. Q14195
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human DPYSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-94
1:2 Average % 106
Range % 103-109
1:4 Average % 85
Range % 81-89
1:8 Average % 94
Range % 90-98
The recovery of human DPYSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 80-88
EDTA plasma (n=4) 95 91-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.275 2.198 2.237 2.145
1000 1.772 1.802 1.787 1.695
500 1.308 1.300 1.304 1.212
250 0.902 1.000 0.951 0.859
125 0.645 0.656 0.651 0.559
62.5 0.441 0.438 0.440 0.348
31.25 0.303 0.310 0.307 0.215
0 0.091 0.092 0.092  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
Gene References into Functions
  1. Data indicate that CRMP4a and CRMP4b isoforms have opposite effects on cell proliferation, migration, and invasion in gastric cancer. CRMP4a expression was reduced in gastric carcinoma tissues and cell lines and seems to act as a tumor suppressor in gastric carcinoma. CRMP4b expression was higher in tumor tissue and cell lines. PMID: 27475326
  2. DPYSL3 expression levels inversely correlated with those of VEGF and FAK. Down-regulation of DPYSL3 expression in HCC tissues may serve as a predictive biomarker for HCC after curative resection. PMID: 25173447
  3. Expression status of DPYSL3 in gastric cancer tissues may represent a promising biomarker for the malignant behavior of GC. PMID: 25096402
  4. DPYSL3 as pancreatic ductal adenocarcinoma-associated molecule that regulates cell adhesion and migration by stabilization of focal adhesion complex PMID: 24339867
  5. structures provide further insight into the effects of medically relevant mutations of the DPYSL-3 gene encoding CRMP-4 and the putative enzymatic activities of CRMPs PMID: 24914979
  6. High collapsin response mediator protein 4 expression is associated with liver metastasis in pancreatic cancer. PMID: 22805864
  7. Downregulation of MYCN by small interfering RNA (siRNA) increased DPYSL3 levels, while upregulation of MYCN in non-MYCN NB cells decreased DPYSL3 levels. PMID: 24011394
  8. study found the association between the rs147541241 variant and amyotrophic lateral scerosis was limited to the French population PMID: 23568759
  9. Ulip1 was downregulated and HIF1a was upregulated in hypoxic neuroblastoma cells. PMID: 20977846
  10. CRMP4 is a key physiological substrate of GSK3 in regulating chromosomal alignment and mitotic progression through its effect on spindle microtubules PMID: 21179545
  11. Data show that two genes, LTB4DH and DPYSL3, were confirmed to be candidate genes for the predictor of a good immune response. PMID: 20804502
  12. new function of CRMP4 as a metastasis-suppressor in lymph node metastasis of prostate cancer PMID: 20543870
  13. HUlip was expressed in late fetal and early postnatal brains in the neurons of the brain stem, basal ganglia/thalamus, and dentate gyrus of the hippocampus. HUlip expression was easily detected in tumor cells undergoing neuronal differentiation. [Ulip] PMID: 15933812

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Subcellular Location Cytoplasm, Cell projection, growth cone
Protein Families Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family
Tissue Specificity Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Database Links

HGNC: 3015

OMIM: 601168

KEGG: hsa:1809

STRING: 9606.ENSP00000343690

UniGene: Hs.519659


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