Human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit

Code CSB-E11790h
Size 96T,5×96T,10×96T
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Product Details

Target Name
dihydropyrimidinase-like 3
Alternative Names
Collapsin response mediator protein 4 ELISA Kit; Collapsin response mediator protein 4 long ELISA Kit; CRMP 4 ELISA Kit; CRMP-4 ELISA Kit; CRMP4 ELISA Kit; Dihydropyrimidinase like 3 ELISA Kit; Dihydropyrimidinase related protein 3 ELISA Kit; Dihydropyrimidinase-related protein 3 ELISA Kit; DPYL3_HUMAN ELISA Kit; DPYSL3 ELISA Kit; DRP 3 ELISA Kit; DRP-3 ELISA Kit; DRP3 ELISA Kit; LCRMP ELISA Kit; TUC4 ELISA Kit; ULIP 1 ELISA Kit; ULIP ELISA Kit; ULIP-1 ELISA Kit; ULIP1 ELISA Kit; UNc 33 like phosphoprotein 1 ELISA Kit; Unc 33 like phosphoprotein ELISA Kit; Unc-33-like phosphoprotein 1 ELISA Kit
Abbreviation
DPYSL3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DPYSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-94
1:2 Average % 106
Range % 103-109
1:4 Average % 85
Range % 81-89
1:8 Average % 94
Range % 90-98
Recovery
The recovery of human DPYSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 80-88
EDTA plasma (n=4) 95 91-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.275 2.198 2.237 2.145
1000 1.772 1.802 1.787 1.695
500 1.308 1.300 1.304 1.212
250 0.902 1.000 0.951 0.859
125 0.645 0.656 0.651 0.559
62.5 0.441 0.438 0.440 0.348
31.25 0.303 0.310 0.307 0.215
0 0.091 0.092 0.092  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DPYSL3 ELISA Kit was designed for the quantitative measurement of Human DPYSL3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

Function
(From Uniprot)
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Gene References into Functions
  1. Data indicate that CRMP4a and CRMP4b isoforms have opposite effects on cell proliferation, migration, and invasion in gastric cancer. CRMP4a expression was reduced in gastric carcinoma tissues and cell lines and seems to act as a tumor suppressor in gastric carcinoma. CRMP4b expression was higher in tumor tissue and cell lines. PMID: 27475326
  2. DPYSL3 expression levels inversely correlated with those of VEGF and FAK. Down-regulation of DPYSL3 expression in HCC tissues may serve as a predictive biomarker for HCC after curative resection. PMID: 25173447
  3. Expression status of DPYSL3 in gastric cancer tissues may represent a promising biomarker for the malignant behavior of GC. PMID: 25096402
  4. DPYSL3 as pancreatic ductal adenocarcinoma-associated molecule that regulates cell adhesion and migration by stabilization of focal adhesion complex PMID: 24339867
  5. structures provide further insight into the effects of medically relevant mutations of the DPYSL-3 gene encoding CRMP-4 and the putative enzymatic activities of CRMPs PMID: 24914979
  6. High collapsin response mediator protein 4 expression is associated with liver metastasis in pancreatic cancer. PMID: 22805864
  7. Downregulation of MYCN by small interfering RNA (siRNA) increased DPYSL3 levels, while upregulation of MYCN in non-MYCN NB cells decreased DPYSL3 levels. PMID: 24011394
  8. study found the association between the rs147541241 variant and amyotrophic lateral scerosis was limited to the French population PMID: 23568759
  9. Ulip1 was downregulated and HIF1a was upregulated in hypoxic neuroblastoma cells. PMID: 20977846
  10. CRMP4 is a key physiological substrate of GSK3 in regulating chromosomal alignment and mitotic progression through its effect on spindle microtubules PMID: 21179545
  11. Data show that two genes, LTB4DH and DPYSL3, were confirmed to be candidate genes for the predictor of a good immune response. PMID: 20804502
  12. new function of CRMP4 as a metastasis-suppressor in lymph node metastasis of prostate cancer PMID: 20543870
  13. HUlip was expressed in late fetal and early postnatal brains in the neurons of the brain stem, basal ganglia/thalamus, and dentate gyrus of the hippocampus. HUlip expression was easily detected in tumor cells undergoing neuronal differentiation. [Ulip] PMID: 15933812

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Subcellular Location
Cytoplasm. Cell projection, growth cone.
Protein Families
Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family
Tissue Specificity
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Database Links

HGNC: 3015

OMIM: 601168

KEGG: hsa:1809

STRING: 9606.ENSP00000343690

UniGene: Hs.519659

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