Product Details

Purity

>85% (SDS-PAGE)

Target Names

DPYSL3

Uniprot No.

Q14195

Alternative Names

Collapsin response mediator protein 4; Collapsin response mediator protein 4 long; CRMP 4; CRMP-4; CRMP4; Dihydropyrimidinase like 3; Dihydropyrimidinase related protein 3; Dihydropyrimidinase-related protein 3; DPYL3_HUMAN; DPYSL3; DRP 3; DRP-3; DRP3; LCRMP; TUC4; ULIP 1; ULIP; ULIP-1; ULIP1; UNc 33 like phosphoprotein 1; Unc 33 like phosphoprotein; Unc-33-like phosphoprotein 1

Species

Homo sapiens (Human)

Expression Region

1-570

Target Protein Sequence

MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI VPGGVKTIEA NGKMVIPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG TTMIIDHVVP EPESSLTEAY EKWREWADGK SCCDYALHVD ITHWNDSVKQ EVQNLIKDKG VNSFMVYMAY KDLYQVSNTE LYEIFTCLGE LGAIAQVHAE NGDIIAQEQT RMLEMGITGP EGHVLSRPEE LEAEAVFRAI TIASQTNCPL YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA IPEGTNGVEE RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR ISVGSDSDLV IWDPDAVKIV SAKNHQSAAE YNIFEGMELR GAPLVVICQG KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK RIKARRKMAD LHAVPRGMYD GPVFDLTTTP KGGTPAGSAR GSPTRPNPPV RNLHQSGFSL SGTQVDEGVR SASKRIVAPP GGRSNITSLS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

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■ Q&A

I was hoping for a buffer without glycerol, or for them to be lyophilized. Do you know if these proteins can be provided in the Tris-based buffer without glycerol, or would you recommend they be lyophilized?

Thanks for your inquiry. For liquid protein: Glycerol is necessary, we don't recommend to ship the protein in buffer (liquid form) without any glycerol. The glycerol content can be adjusted according to customer's demand, generally 5%-50% is OK.
Glycerol has a protective effect on proteins. First, it can increase the stability of the protein (reducing the polarity of the buffer and preventing hydrophobic aggregation of the protein).
Second, it can reduce the freezing point of the system and facilitate the preservation of the protein at low temperatures, especially when the protein is repeatedly frozen and thawed.
protein damage (aggregation or inactivation) can be avoided as much as possible. As for the final concentration of glycerol, in principle, the final concentration of 5% -50% has no problem,
it may involve in the preservation of time, high glycerol content is relatively conducive to longer storage. However, the fianl content can be adjusted according to the customer's request,
If the customer insists not to add any glycerol, we can also meet your demand, but we should disclaim in advance that without addition of glycerol may lead to protein instability, especially the protein itself is unstable.
For lyophilized protein: There isn't any glycerol added before or after the lyophilization.
Therefore we recommend that customer can choose lyophilized protein if he has strict requirement on the glycerol.

Target Background

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Gene References into Functions

Data indicate that CRMP4a and CRMP4b isoforms have opposite effects on cell proliferation, migration, and invasion in gastric cancer. CRMP4a expression was reduced in gastric carcinoma tissues and cell lines and seems to act as a tumor suppressor in gastric carcinoma. CRMP4b expression was higher in tumor tissue and cell lines. PMID: 27475326
DPYSL3 expression levels inversely correlated with those of VEGF and FAK. Down-regulation of DPYSL3 expression in HCC tissues may serve as a predictive biomarker for HCC after curative resection. PMID: 25173447
Expression status of DPYSL3 in gastric cancer tissues may represent a promising biomarker for the malignant behavior of GC. PMID: 25096402
DPYSL3 as pancreatic ductal adenocarcinoma-associated molecule that regulates cell adhesion and migration by stabilization of focal adhesion complex PMID: 24339867
structures provide further insight into the effects of medically relevant mutations of the DPYSL-3 gene encoding CRMP-4 and the putative enzymatic activities of CRMPs PMID: 24914979
High collapsin response mediator protein 4 expression is associated with liver metastasis in pancreatic cancer. PMID: 22805864
Downregulation of MYCN by small interfering RNA (siRNA) increased DPYSL3 levels, while upregulation of MYCN in non-MYCN NB cells decreased DPYSL3 levels. PMID: 24011394
study found the association between the rs147541241 variant and amyotrophic lateral scerosis was limited to the French population PMID: 23568759
Ulip1 was downregulated and HIF1a was upregulated in hypoxic neuroblastoma cells. PMID: 20977846
CRMP4 is a key physiological substrate of GSK3 in regulating chromosomal alignment and mitotic progression through its effect on spindle microtubules PMID: 21179545
Data show that two genes, LTB4DH and DPYSL3, were confirmed to be candidate genes for the predictor of a good immune response. PMID: 20804502
new function of CRMP4 as a metastasis-suppressor in lymph node metastasis of prostate cancer PMID: 20543870
HUlip was expressed in late fetal and early postnatal brains in the neurons of the brain stem, basal ganglia/thalamus, and dentate gyrus of the hippocampus. HUlip expression was easily detected in tumor cells undergoing neuronal differentiation. [Ulip] PMID: 15933812

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Subcellular Location

Cytoplasm. Cell projection, growth cone.

Protein Families

Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family

Tissue Specificity

Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

Database Links

HGNC: 3015

OMIM: 601168

KEGG: hsa:1809

STRING: 9606.ENSP00000343690

UniGene: Hs.519659

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Recombinant Human Dihydropyrimidinase-related protein 3 (DPYSL3)

Product Details

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Target Background

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