Rabbit C-Reactive Protein,CRP ELISA Kit

Code CSB-E06847Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
C-reactive protein, pentraxin-related
Alternative Names
CRP ELISA kit; PTX1C-reactive protein ELISA kit
Abbreviation
CRP
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma
Detection Range
7.8 pg/mL-2000 pg/mL
Sensitivity
2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 96  
Range % 90-102  
1:2000 Average % 91  
Range % 82-96  
1:4000 Average % 90  
Range % 85-98  
1:8000 Average % 94  
Range % 83-98  
Recovery
The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 90-104  
EDTA plasma (n=4) 91 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.196 0.198 0.197    
500 0.353 0.371 0.362    
125 0.669 0.651 0.660    
31.3 1.084 1.035 1.060    
7.8 2.018 1.974 1.996    
0 2.913 3.122 3.018    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit CRP ELISA Kit was designed for the quantitative measurement of Rabbit CRP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-2000 pg/mL and the sensitivity is 2 pg/mL.

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Target Background

Function
(From Uniprot)
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Gene References into Functions
  1. Rabbit CRP acute-phase system binds phosphocholine and phosphoethanolamine. PMID: 23600950
  2. IL-6 augments mechanical strain-induced CRP synthesis in vessels via the stretch-activated channel-NF-kappaB pathway. PMID: 23257175
  3. CRP antagonism of eNOS is mediated by coupling of FcgammaRI to FcgammaRIIB by Src kinase and activation of inositol 5'-phosphatase 1, and consistent with this mechanism, both FcgammaRI and FcgammaRIIB are required for CRP to blunt endothelial repair in vivo. PMID: 21940940
  4. Metformin attenuated the progression of aortic atherosclerosis via down-regulation of serum C-reactive protein. PMID: 20108078
  5. During abdominal aortic aneurysm formation, increased serum hsCRP levels derive from aneurysmal arteries with degenerating elastic lamina. PMID: 19502992

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Subcellular Location
Secreted.
Protein Families
Pentraxin family
Tissue Specificity
Found in plasma.
Database Links
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