Rabbit C-Reactive Protein,CRP ELISA Kit

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Rabbit C-Reactive Protein,CRP ELISA Kit

Citations (4) Protocol

Code	CSB-E06847Rb
Size	96T,5×96T,10×96T
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Citations (4)
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Description

This Rabbit CRP ELISA Kit was designed for the quantitative measurement of Rabbit CRP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-2000 pg/mL and the sensitivity is 2 pg/mL.

Target Name

C-reactive protein, pentraxin-related

Alternative Names

CRP ELISA kit; PTX1C-reactive protein ELISA kit

Abbreviation

CRP

Uniprot No.

P02742

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma

Detection Range

7.8 pg/mL-2000 pg/mL

Sensitivity

2 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	90-102
1:2000	Average %	91
	Range %	82-96
1:4000	Average %	90
	Range %	85-98
1:8000	Average %	94
	Range %	83-98

Recovery

The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.196	0.198	0.197
500	0.353	0.371	0.362
125	0.669	0.651	0.660
31.3	1.084	1.035	1.060
7.8	2.018	1.974	1.996
0	2.913	3.122	3.018

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Mesenchymal stem cells significantly improved treatment effects of Linezolid on severe pneumonia in a rabbit model Kong D, et al,Bioscience Reports,2019
Effect(s) of Long-Term Anaesthesia Induced by Isoflurane, Sevoflurane, Propofol-Fentanyl, Medetomidin-Midozolam-Ketamine or Xylazine-Ketamine Combinations on the Acut Phase Proteins and Cardiac Troponins Levels in Rabbits Nuh Kilic.et al,/,2018
Experimental Study of Phlebitis Ointment Administration in Acute Superficial Thrombophlebitis Guangzong Li.et al,EVID-BASED COMPL ALT,2018
Baccaurea angulata fruit juice reduces atherosclerotic lesions in diet-induced Hypercholesterolemic rabbits Ibrahim M.et al,Lipids Health Dis,2017

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Function (From Uniprot)	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Gene References into Functions	Rabbit CRP acute-phase system binds phosphocholine and phosphoethanolamine. PMID: 23600950 IL-6 augments mechanical strain-induced CRP synthesis in vessels via the stretch-activated channel-NF-kappaB pathway. PMID: 23257175 CRP antagonism of eNOS is mediated by coupling of FcgammaRI to FcgammaRIIB by Src kinase and activation of inositol 5'-phosphatase 1, and consistent with this mechanism, both FcgammaRI and FcgammaRIIB are required for CRP to blunt endothelial repair in vivo. PMID: 21940940 Metformin attenuated the progression of aortic atherosclerosis via down-regulation of serum C-reactive protein. PMID: 20108078 During abdominal aortic aneurysm formation, increased serum hsCRP levels derive from aneurysmal arteries with degenerating elastic lamina. PMID: 19502992 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Pentraxin family
Tissue Specificity	Found in plasma.
Database Links	KEGG: ocu:100009091 STRING: 9986.ENSOCUP00000004059 UniGene: Ocu.1848

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St. Ste 610-312, Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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