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Recombinant Streptococcus sp Streptokinase G protein (skg) (Active)

Recombinant Streptococcus sp Streptokinase G protein (skg) (Active)

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Code	CSB-AP000171SNC
Abbreviation	Recombinant Streptococcus sp. skg protein (Active)
MSDS	MSDS Datasheet
Size	$176
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Product Details
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Target Background

Product Details

Purity

≥97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8.0x10⁴ IU/mg.

Target Names

skg

Uniprot No.

P10519

Research Area

Cardiovascular?

Alternative Names

skg; Streptokinase G

Species

Streptococcus sp. (strain 19909)

Source

E.Coli

Expression Region

27-440aa

Complete Sequence

IAGPEWLLDR PSVNNSQLVV SVAGTVEGTN QDISLKFFEI DLTSRPAHGG KTEQGLSPKS KLFATDSGAM PHKLEKADLL KAIQEQLIAN VHSNDDYFEV IDFASDATIT DRNGKVYFAD KDGSVTLPIQ PVQEFLLKGH VRVRPYKEKP VQNQAKSVDV EYTVQFTPLN PDDDFRPALK DTKLLKTLAI GDTITSQELL AQAQSILNKN HPGYTIYERD SSIVTHDNDI FRTILPMDQE FTYHVKNREQ AYRINKKSGL NEEINNTDLI SEKYYVLKKG EKPYDPFDRS HLKLFTIKYV DVNTNELLKS EQLLTASERN LDFRDLYDPR DKAKLLYNNL DAFGIMDYTL TGKVEDNHDD TNRIITVYMG KRPEGENASY HLAYDKDRYT EEEREVYSYL RYTGTPIPDN PNDK

Mol. Weight

47.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Streptokinase G activates plasminogen to initiate fibrinolysis, making it a critical tool for cardiovascular research focused on thrombolytic mechanisms. This tag-free recombinant form, covering residues 27–440 of the mature protein, ensures the plasminogen-binding interface remains unobstructed—a prerequisite for accurate kinetic parameter analysis (Km, Vmax, kcat) and inhibitor screening with reliable IC50 determination. The specific activity of 8.0 × 10⁴ IU/mg, validated by fibrin lysis in an agarose plate assay, confirms full biological function and supports use as a quantitative positive control in enzyme-linked fibrinolytic assays or substrate specificity profiling experiments. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for drug candidate evaluation studies and provides a suitable basis for structural investigations such as crystallography or cryo-EM.

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Target Background

Function

This protein is not a protease, but it activates plasminogen by complexing with it. As a potential virulence factor, it is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells.

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