Co-Immunoprecipitation (Co-IP)Kit(Animal-derived Sample)

Antibodies are bound to the corresponding proteins in the lysate, then incubated with ProteinA/G coupled MagneticBeads, and the ProteinA/G bead-antibody-destination protein complex is obtained by centrifugation or with the help of a magnetic rack. Under the action of high temperature and reducing agent, the antigen and antibody are dissociated, and the supernatant is collected, which includes the antibody, destination protein and a small amount of heteroprotein. The supernatant includes antibody, target protein and a small amount of heterogeneous protein, and then the protein is identified by Western Blot or mass spectrometry (MS).

Notes：1. You need to prepare your own PBS, 6 Xloading buffer, magnetic rack and other reagents and consumables.

             2. 6T is 6 times single group (1 group IP or 1 group IgG) immunoprecipitation experiments, the latter operation step contains 1 group each of 1gG and IP, need to consume 2T reagents.