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Recombinant Human IL12B&IL12A Heterodimer Protein (Active)

Recombinant Human IL12B&IL12A Heterodimer Protein (Active)

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Code	CSB-AP001811HU
Abbreviation	Recombinant Human IL12B&IL12A Heterodimer protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard

Target Names

IL12A

Uniprot No.

P29460 P29459

Research Area

Immunology

Alternative Names

CLMF; CLMF p35; CLMF p40; CLMF1; CLMF2; Cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 2; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12A; IL 2 subunit p40; IL-12 subunit p35; IL-12A; IL-12B; IL12; IL12, p35 subunit; IL12, subunit p40; IL12A; IL12A_HUMAN; IL12B; IL35 subunit; Interleukin 12 alpha chain; Interleukin 12 beta chain; Interleukin 12 subunit beta; Interleukin 12, p40; Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); Interleukin 12B; Interleukin-12 subunit alpha; Natural killer cell stimulatory factor 1, 35 kD subunit; natural killer cell stimulatory factor 2; Natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

23-328aa(IL12B) & 23-219aa(IL12A)

Complete Sequence

p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Mol. Weight

57kDa

Protein Length

Heterodimer

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

0.2 μm filtered PBS, pH 7.4 ,lyophilized

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

IL-12 drives Th1 polarization and NK cell activation through JAK2/TYK2-STAT4 signaling, making it a critical tool for dissecting innate-to-adaptive immune transitions. This tag-free human IL-12 heterodimer (IL12B aa 23–328 and IL12A aa 23–219) demonstrates exceptional potency with an ED50 of 0.01–0.05 ng/mL in PHA-stimulated human T lymphoblast proliferation assays, corresponding to a specific activity of approximately 1.1 × 10⁴ units/μg. The endotoxin level below 1.0 EU/μg satisfies the stringent criteria required to prevent LPS-driven artifacts in sensitive immune cell functional assays, including PBMC proliferation, Th1 differentiation studies, JAK/STAT pathway activation experiments, and in vivo tumor immunology models. Purity exceeding 95% by SDS-PAGE, combined with faithful representation of the mature heterodimer sequence, provides a suitable basis for use as a reference standard in cytokine detection assays or as a positive control in antibody validation workflows.