Code | CSB-MA0049471A0m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:500 |
The CD59 monoclonal antibody is a highly specific tool for the detection of CD59 protein. Its preparation mainly includes five steps: immunization a mouse with the recombinant CD59 protein to isolate the spleen cells, fusion mouse spleen cells with myeloma cells to form hybridomas, selecting CD59 antibody-producing hybridomas, cultuing the selected hybridomas and collecting the desired antibodies. This CD59 monoclonal antibody is purified using protein G affinity chromatography to achieve high purity and specificity. Its specificity has been tested in ELISA, WB, and IHC applications.
CD59 protein, also known as protectin, is a cell surface glycoprotein that plays a crucial role in protecting host cells from the membrane attack complex (MAC) of the complement system. This protects the cell from MAC-mediated damage and maintains the integrity of the cell membrane. It is an important regulator of complement-mediated cell lysis.
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