Wheat Gliadin

Code CSB-NP004501Pl
Product Type Native Protein
Size 16Purchase it in Cusabio online store
(Shop online in the US. Customers outside the US,
please order from local distributors.)
Lead Time 2 weeks
Relevance Gliadins are known for their role, along with glutenin, in the formation of gluten. It is slightly soluble in ethanol and contains only intramolecule disulfide links. These proteins are essential to giving bread the ability to rise properly and fix it\\\'s shape in baking. They are also some of the best examples of food-derived pathogenesis. People with gluten-sensitive enteropathy (the severe form of which is coeliac disease) are sensitive to α, β, and γ gliadins. Those with wheat-dependent (WD) exercise-induced anaphylaxis, WD urticaria and Baker\\\'s asthma are sensitive to ω-gliadins. Gliadin can also serve as a useful delivery method for sensitive enzymes (such as superoxide dismutase, which is fused with gliadin to form glisodin) -- this helps protect them from stomach acids that cause breakdown.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Tested Applications ELISA, WB, SDS-PAGE
Form Liquid
Storage Buffer 70% Ethanol
Alias N/A
Biological_Activity Not test
Purity >95% (SDS-PAGE)
Sequence Full length protein
Research Area Cell Biology
Source Purified from Wheat seed
Protein Names Wheat Gliadin
Storage Aliquot and store at 25°C.
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Q&A and Customer Reviews

Q&A
Q:

I would like to use this protein as a control for acid-PAGE.
Is this preparation suitable for this kind of application and do you have any recommendations for its use? Do you know the MW that can be expected for acid-PAGE of this protein?

A:
Thanks for your inquiry. This protein is suitable for the application of acid-PAGE, however, as we haven't this kind of application for the time being,
we can't provide any useful information, including the MW that can be expected for acid-PAGE of this protein.
But we have conducted SDS-PAGE detection, please refer to the following link of the image:
https://www.cusabio.com/Native-Protein/Wheat-Gliadin-118654.html
Q:

Is this product supplied as a clear liquid or does it have a color to it or is it hazy?

A:
Thanks for your inquiry. CSB-NP004501Pl Wheat Gliadin
This protein is supplied as a light yellow liquid. The storage temperature is room temperature, because it is easy to form precipitates under low temperature.
If there appears precipitates, the precipitates can be re-dissolved with the same buffer.
Q:

Regarding your protein CSB-NP004501Pl, could you please advise on the concentration of the current lot?

A:
Very nice to receive your inquiry. The concentration is generally 10-50mg/ml, we can adjust according to your requirement if you have any special request.
So please remark your requirement for the concentration when you place the order.
Q:

What is the concentration? Is it suitable as standard for Elisa?

A:
Thanks for your inquiry.
The concentration is 20-40mg/ml.
It is a mixed protein, it is not recommended as standard for Elisa.
Q:

Would you happen to know the concentration of the current lot on hand?

A:
Very nice to receive your inquiry. The current concentration is 20-40mg/ml. This is a mixed protein and we do not recommend it as a standard for EILSA.
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