Recombinant Human Claudin-9 (CLDN9)-VLPs (Active)

This Recombinant Human Claudin-9 (CLDN9) is a full-length, functionally active antigen presented using Virus-Like Particle (VLP) technology. The key advantage of this VLP platform is its ability to present the complete native extracellular structure of CLDN9 (Met 1-Val 217) in its authentic, mammalian cell-derived conformation, which is critical for maintaining correct epitope presentation. This is rigorously validated by functional data: in a binding ELISA, the immobilized CLDN9-VLPs exhibit specific, high-affinity interaction with an anti-CLDN6/9 recombinant antibody, yielding an EC₅₀ of 24.93-35.47 ng/mL, as demonstrated by a clear sigmoidal dose-response curve. The product's integrity and purity are confirmed by Western Blot analysis under denaturing conditions, which shows a single, sharp band about 25 kDa detected by an anti-6*His antibody, corresponding to the expected size of the 24.2 kDa, C-terminal 10xHis-tagged protein. With endotoxin levels below 1.0 EU/µg, this reagent provides a superior, native-like immunogen with validated binding activity for advanced antibody development and immunoassays targeting CLDN9.