Recombinant Human Claudin-9(CLDN9)-VLPs (Active)

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Code CSB-MP005511HU
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  • CSB-MP005511HU is detected by Mouse anti-6*His monoclonal antibody.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL. Biological Activity Assay
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Product Details

Description

This Human Claudin-9 (CLDN9) recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human CLDN9 (1-217aa) was expressed with the C-terminal 10xHis tag. The activity was validated.
In addition, this Human CLDN9 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.

CLDN9 (Claudin-9) is located on human chromosome 16p13.3, with a full length of 651 bases. The CLDN9 gene is overexpressed in the blood, pituitary and brain, and is highly expressed in endometrial cancer, liver cancer and gastric cancer cell lines. CLDN9 protein is mainly Localized at cell tight junctions, cell membranes and channel proteins.
CLDN9 is a tight junction protein. Abnormal expression of CLDN9 will lead to impaired tight junctions function and reduced barrier function, resulting in increased tissue permeability, which will eventually lead to many diseases including hereditary, allergic diseases, infectious diseases of various systems and even tumors. Therefore, CLDN9 is regarded as a potential target for the treatment of cancer or other diseases.

Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL.
Target Names CLDN9
Uniprot No. O95484
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 1-217aa
Target Protein Sequence MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV
Mol. Weight 24.2 kDa
Protein Length Full Length
Tag Info C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Gene References into Functions
  1. CLDN9 may be an important biomarker for invasive pituitary oncocytomas. PMID: 25281028
  2. Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis. PMID: 19960275
  3. Mouse studies suggest that claudin-9 deficiency may cause hearing loss not only in mice but also in humans. PMID: 19696885
  4. Residues N38 and V45 in the first extracellular loop (EL1) of CLDN9 are necessary for HCV entry. PMID: 17804490
  5. CLDN9, clustered with CLDN6 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707

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Subcellular Location Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families Claudin family
Tissue Specificity Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.
Database Links

HGNC: 2051

OMIM: 615799

KEGG: hsa:9080

STRING: 9606.ENSP00000398017

UniGene: Hs.296949

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