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Recombinant Human Ephrin-A4 (EFNA4), partial (Active)

Recombinant Human Ephrin-A4 (EFNA4), partial (Active)

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Code	CSB-AP005461HU
Abbreviation	Recombinant Human EFNA4 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$96
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Target Background

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human EphA7-His at 2μg/ml can bind Human EFNA4-Fc-His, the ED50 of Recombinant Human EFNA4-Fc-His is 1.5190 ug/ml.

Target Names

EFNA4

Uniprot No.

P52798

Research Area

Cardiovascular

Alternative Names

EFL 4; EFL4; EFN A4; EFNA 4; EFNA4; EFNA4_HUMAN; Eph related receptor tyrosine kinase ligand 4; EPH-related receptor tyrosine kinase ligand 4; Ephrin-A4; EphrinA4; EPLG 4; EPLG4; LERK 4; LERK-4; LERK4; Ligand of Eph related kinase 4; MGC125826

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

26-171aa

Complete Sequence

LRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERKSESAHPVGSPGESG

Mol. Weight

44.3 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-hFc-tagged

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered PBS, pH 7.4.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Datasheet & COA

Please contact us to get it.

Description

Ephrin-A4 plays a critical role in Eph receptor-mediated signaling that governs vascular development, cell migration, and axon guidance, making it a key target in cardiovascular and neurobiology research. This recombinant human EFNA4 covers residues 26–171 of the mature ectodomain, is expressed in mammalian cells to preserve native-like glycosylation, and carries a C-terminal His-Fc tag that facilitates receptor-ligand binding and competition assays. Functional ELISA validation demonstrates an ED50 of approximately 1.52 µg/ml against immobilized human EphA7, confirming receptor-binding potency suitable for SPR-based interaction studies, antibody characterization, and ELISA standard curve development. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/µg satisfies the criteria typical for cell-based proliferation and survival assays, angiogenesis models, and in vivo tumor biology studies where contaminant interference must be minimized.

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Target Background

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.

Gene References into Functions

Study present for the first time in vitro and in vivo evidence suggesting that the major role of two ephrin A4 isoforms in chronic lymphocytic leukemia could be related with a non-previously described mechanism of survival linked to extravasation strongly dependent on integrin signaling. PMID: 27374180
The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy. PMID: 20071790
Characterization of the promoter PMID: 12030849
This provides genetic evidence that Twist1, Msx2 and Efna4 function together in boundary formation and the pathogenesis of coronal synostosis. PMID: 16540516
CLL B-cells showed a more heterogeneous Eph/EFN profile, specially EFNA4, EphB6 and EphA10. EphB6 and EFNA4 were further related with the clinical course of CLL. PMID: 18819711
EFNA4-EphA2 interactions are involved in Chronic lymphocytic leukemia cell trafficking between blood and the tissues PMID: 19828693

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Subcellular Location

[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 2]: Secreted.

Protein Families

Ephrin family

Tissue Specificity

Expressed in the adult spleen, lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liver and kidney. Also detected in hematopoietic cell lines.

Database Links

HGNC: 3224

OMIM: 601380

KEGG: hsa:1945

STRING: 9606.ENSP00000414378

UniGene: Hs.449913

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