Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of >333 IU/mg.
Alternative Names
AA517111; Basement membrane protein 40; Basement-membrane protein 40; BM 40; BM-40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPARC; SPRC; SPRC_HUMAN
Species
Homo sapiens (Human)
Expression Region
18-303aa
Complete Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMGSA+P QQEALPDETE VVEETVAEVT
EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN
TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH
KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT
EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD
QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC
FGIKQKDIDK DLVI
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Human SPARC is a premium-grade, N-terminal 6xHis-tagged full-length protein that corresponds to the 18-303aa expression region of the Secreted protein acidic and rich in cysteine (SPARC). Designed for use in cancer research, this protein is expressed in E. coli and features exceptional purity, exceeding 95% as determined by both SDS-PAGE and HPLC. With endotoxin levels below 1.0 EU/µg, as determined by the LAL method, this product ensures minimal interference in experimental applications.
Recombinant Human SPARC is fully biologically active when compared to the standard. Its ED50, ascertained by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells, is less than 3.0 µg/mL. This corresponds to a specific activity of > 333 IU/mg, demonstrating the protein's effectiveness in various research contexts. The lyophilized powder form ensures convenient storage and utilization across a range of experiments.