Recombinant Human SPARC protein(SPARC) (Active)

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Code CSB-AP000311HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of >333 IU/mg.
Target Names SPARC
Uniprot No. P09486
Research Area Cancer
Alternative Names AA517111; Basement membrane protein 40; Basement-membrane protein 40; BM 40; BM-40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPARC; SPRC; SPRC_HUMAN
Species Homo sapiens (Human)
Source E.coli
Expression Region 18-303aa
Complete Sequence MSYYHHHHHH DYDIPTTENL YFQGAMGSA+P QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI
Mol. Weight 36.1 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Gene References into Functions
  1. Hepatic SPARC expression is associated with liver injury and fibrogenic processes in non-alcoholic fatty liver disease. PMID: 29335425
  2. Plasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p PMID: 30227835
  3. The authors have confirmed the presence of SPARC expression in melanoma, Kaposi sarcomas (KS), leiomyosarcomas (LMS) and angiosarcomas (AS) and also detected it for the first time in atypical fibroxanthomas (AFX). PMID: 29660567
  4. Results revealed that hypermethylation of the SPARC promoter was the primary mechanism of SPARC downregulation in prostate cancer. SPARC expression was frequently lost during the promoter hypermethylation but could be restored by 5-Aza-Cdr. PMID: 29207175
  5. Stromal SPARC expression was a useful biomarker for predicting prognosis in patients with resected pancreatic ductal adenocarcinoma. PMID: 29295776
  6. SPARC is closely related to the development of breast cancer and can be used as a tumor marker for breast cancer recurrence. PMID: 29237913
  7. SPARC expression was inversely associated with the degree of malignancy and it had a negative correlation with VEGF-C and VEGF-D expression. Results suggest SPARC might function as a tumor suppressor inhibiting angiogenesis and lymphangiogenesis in ovarian cancer by reducing the expression of VEGF-C and VEGF-D. PMID: 29075785
  8. SPARC may be involved in gastric cancer metastasis by effecting on tumor microenvironment PMID: 29077165
  9. SPARC treatment enhances the epithelial mesenchymal transition signaling pathway via activation of AKT, and exogenous SPARC and tumor expressing SPARC might be associated with tumor progression in head and neck cancers. PMID: 28718842
  10. The epicardial adipose tissue expresses the mRNA of osteopontin, osteoprotegerin, and osteonectin genes that have been implicated in the calcification process; such expression is statistically associated with some components of HDL subclasses in coronary artery disease patients. PMID: 28821297
  11. detection of SPARC mRNA and protein expression levels may facilitate early diagnosis and prognosis assessment of esophageal squamous cell carcinoma. PMID: 28713937
  12. SPARC rs17718347 and rs2347128 single nucleotide polymorphisms are associated with progression-free survival in locally advanced and metastatic pancreatic cancer patients. PMID: 28687963
  13. results demonstrated that loss of miR-211 expression and thus uncontrolled SPARC overexpression might drive progression of hepatocellular carcinoma (HCC), which may provide a novel therapeutic strategy for the treatment of HCC. PMID: 27230656
  14. Data suggest that plasma SPARC levels may be biomarker for vascular complications among Chinese type 2 diabetic patients; patients in lowest SPARC tertile have increased odds of aortic stiffness but reduced odds of peripheral arterial disease. PMID: 28479157
  15. The profiled circulating tumour cells also expressed elevated levels of stem cell markers, and the extracellular matrix protein, SPARC. The expression of SPARC might correspond to an epithelial-mesenchymal transition in pancreatic circulating tumour cells PMID: 28569190
  16. Data suggest that both osteonectin and FGF21 levels in serum are associated with early nephropathy in type 2 diabetes, albeit with different patterns; persistent hyperglycemia may inhibit bone formation leading to osteoporosis. (FGF21 = fibroblast growth factor 21) PMID: 27916484
  17. These results suggest that increased SPARC expression may be an indicator of greater aggressiveness, and may serve as a prognostic factor for triple-negative breast cancer. PMID: 27421134
  18. High expression of SPARC is related to worse prognosis in rectal cancer patients. PMID: 28009327
  19. Tumors with stroma-derived SPARC displayed suppressed growth, inhibited angiogenesis and increased lipid accumulation. Based on the described chaperone function of SPARC, authors hypothesized that SPARC binds albumin complexed with fatty acids and transports them to tumors. PMID: 27776337
  20. Weekly NAB-paclitaxel might be effective for heavily pretreated non-small-cell lung cancer patients. SPARC expression in tumor stroma cells might be a potential negative predictor of NAB-paclitaxel. PMID: 28304139
  21. SPARC-associated signaling pathways are associated with lymphangiogenesis and lymph node metastases of hypopharyngeal cancer. PMID: 29374693
  22. Stromal SPARC expression correlated with the prognosis of patients with resectable biliary carcinoma, and its significance was enhanced in patients treated with adjuvant gemcitabine-based chemotherapy. PMID: 28342122
  23. Studies revealed that SPARC plays a critical role in regulating bone remodeling and maintaining bone mass and quality. The mechanisms by which SPARC influences bone formation, maintenance, and repair might occur through multiple pathways that include the regulation of procollagen processing and assembly in the bone matrix, crosslinking, mineralization, and/or osteoblast/osteoclast differentiation and activity. [review] PMID: 26851678
  24. This study indicates that germline PTCH1 heterozygous mutations play a major role in bone metabolism in patients with NBCCS, in particular in those with PTCH1 protein truncation mutations. SPARC may represent an important downstream modulator of PTCH1 mediation of bone metabolism. PMID: 26890308
  25. The SPARC drives pathological responses in non-small cell lung cancer and idiopathic pulmonary fibrosis by promoting microvascular remodelling and excessive deposition of ECM proteins. PMID: 27759879
  26. proCOL11A1, fibroblast-activated protein, secreted protein acidic and rich in cysteine, and periostin expression was significantly increased in the intratumoral stroma of pancreatic ductal adenocarcinomas compared to paired non-neoplastic pancreata PMID: 29025374
  27. The mRNA and protein levels of SPARC were 5.78-fold higher in cancer tissues compared with the case-matched normal epithelium. High expression levels of SPARC in esophageal squamous cell carcinoma parenchyma were related to lymph node metastasis and poor prognosis (p = 0.049 and p = 0.04). PMID: 28818666
  28. SPARC can serve a dual function role as both predictor for prognosis and potentially biomarker for lymph node metastasis in resected pancreatic cancer patients. PMID: 28119265
  29. SPARC appears to be an important modulator of the actin cytoskeleton, implicating maintenance of muscular function PMID: 27908613
  30. that SPARC-mediated degradation of the extracellular matrix, and its possible association with MMPs, might contribute to progression of phyllodes tumors PMID: 27909812
  31. WIN-dependent increase in the level of SPARC plays a critical role in sensitizing osteosarcoma cells to TRAIL action PMID: 26698404
  32. high SPARC-expression was a significant predictor of poor OS in HPV-negative OPSCC patients using Kaplan-Meier analysis and the log-rank test PMID: 26523779
  33. We identified five new biomarkers: GDF15, osteonectin, TRAP5, TWEAK, and YKL40 as being promising markers for monitoring bone metastases. PMID: 27069189
  34. SPARC levels were not associated with efficacy in patients with MPC. This exploratory analysis does not support making treatment decisions regarding nab-paclitaxel plus gemcitabine or gemcitabine alone in MPC based on SPARC expression. PMID: 26169969
  35. CD90 is upregulated in gastric cancer and inhibits gastric cancer cell apoptosis by modulating the expression level of SPARC protein. PMID: 26329007
  36. SPARC might be an unfavorable indicator in patients with pancreatic cancer, especially in the stroma.[Review] PMID: 26731428
  37. tracheal aspirate SPARC levels predicted development of bronchopulmonary dysplasia or death. PMID: 26656750
  38. SPARC is associated with carcinogenesis of oral squamous epithelium. PMID: 25311631
  39. Studies indicate that SPARC (secreted protein acidic and rich in cysteine) gene is involved in the development and progression of pancreatic ductal adenocarcinoma (PDAC). PMID: 26335014
  40. MLKL upregulation in SPARC overexpressed cells treated with Ara-C, indicates necrosis as a possible cell death process for the SKM-1 cells under these stringent conditions. PMID: 26165695
  41. up-regulation of SPARC by oxLDL is independent of Runx2, and it may be mediated by other transcription factors PMID: 26025968
  42. Tumor-produced SPARC and VCAM1 are regulators of cancer extravasation. PMID: 25925867
  43. miR-29a and miR-29b enhance cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. PMID: 25786138
  44. SNP1599 differentially regulates osteonectin expression and contributes to variability in bone mass, by a mechanism that may involve differential targeting by miR-433 PMID: 25262637
  45. Reduced expression of SPARC in colorectal cancer tissue is associated with poor prognosis and aggressive clinicopathological features. PMID: 26044224
  46. Three functional SPARC SNPs are associated with an increased risk of coal workers' pneumoconiosis in a Chinese population. PMID: 25126876
  47. reduced expression in the bone marrow biopsy specimen in patients with aplastic anemia PMID: 25032754
  48. SPARC promoter methylation as an important factor in the tumorigenesis of gastric carcinomas. PMID: 25516351
  49. SCD5 impairs SPARC and cathepsin B secretion in human melanoma cells and intracellular pH acidification. PMID: 25802234
  50. High SPARC expression of the primary tumor is associated with a higher chance of achieving a pathological complete remission after TAC or TAC-NX chemotherapy. PMID: 25355716
  51. This study indicates that polymorphisms in SPARC and NLRP2 are related to rheumatoid arthritis susceptibility in a Chinese Han population. PMID: 24754275
  52. Recessive osteogenesis imperfecta caused by missense mutations in SPARC. PMID: 26027498
  53. High SPARC expression is associated with non-small cell lung cancer. PMID: 25374228
  54. The matricellular protein SPARC, secreted by a prostate cancer clonal tumor cell subpopulation displaying non-cancer stem cell properties, is a critical mediator of paracrine effects. PMID: 25331979
  55. A novel function of SPARC in the regulation of glucose metabolism in hepatocellular carcinoma. PMID: 25252848
  56. We conclude that SPARC expression has no effect on progression or invasiveness of pituitary tumors. PMID: 25556322
  57. SPARC expression inperi-tumoralstroma predicts a poor prognosis for patients with biliary tract cancer after surgery PMID: 25155283
  58. Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells. PMID: 24804200
  59. SPARC expression was negatively correlated with clinicopathological factors of gastric cancer PMID: 24676680
  60. These results indicate that promoter methylation status of PCDH10, SPARC, and UCHL1 may be used both as prognostic and predictive molecular marker for colorectal cancer patients PMID: 24309322
  61. SPARC is downregulated in pancreatic cancer cells and retards the growth of pancreatic cancer cell PMID: 25027401
  62. SPARC expression is a predictive marker for response to gemcitabine in pancreatic cancer. PMID: 24562449
  63. SPARC may contribute to the metabolic dysregulation associated with obesity in humans. PMID: 23666856
  64. data strongly support the hypothesis that SPARC is involved in directing melanoma metastases specifically to the lung PMID: 24993904
  65. SPARC acts as an oncogene in transformed myelodysplastic syndrome/acute myeloid leukemia. PMID: 24535175
  66. This is the first report of overexpression of SPARC in endometrial cancer stem-like cells. SPARC expression is associated with cell migration and stroma formation PMID: 24769035
  67. SPARC has a potential role in the pathophysiology of gestational diabetes mellitus. PMID: 24349098
  68. Down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC. PMID: 23904381
  69. We observed a positive correlation of osteonectin expression in connective-type stromal tissue surrounding the epithelial tumor cells of craniopharyngioma with the extent of central nervous system infiltration and recurrence rate PMID: 23648679
  70. The study demonstrated a prognostic value of SPARC, especially with regard to its expression in the stroma surrounding OSCC (P < 0.05 PMID: 24396828
  71. SPARC overexpression is associated with adverse outcome in cytogenetically normal-AML, and secreted SPARC activates the integrin-linked kinase/AKT pathway, likely via integrin and beta-catenin signaling, which is involved in leukemia cell self-renewal. PMID: 24590286
  72. our data identify lovastatin as an important pharmacological suppressor of SPARC expression in TM cells, and provide further insight into the molecular mechanisms mediating statin enhancement of aqueous outflow facility. PMID: 24474275
  73. Overexpression of SPARC in cultured beta-cells resulted in a 2.4-fold increase in insulin secretion in high glucose conditions. PMID: 23840838
  74. coexpression of DCX and SPARC collaboratively diminished radioresistance of glioma cells. PMID: 23846421
  75. SPARC may play an important role in the regulation of normal placentation by promoting the invasion of trophoblast cells into the uterine decidua. PMID: 23935929
  76. Studies indicate that HENT1 and SPARC expression seem to be useful predictive factors of response to chemotherapy in pancreatic cancer. PMID: 23846918
  77. Our findings suggest that SPARC 3'-UTR SNPs may be useful in predicting GA patients at increased risk of recurrence. PMID: 22491017
  78. Results suggest that overexpression of the SPARC gene correlates with tumor invasion depth and survival in gastric cancer. PMID: 24018911
  79. Data indicat that the SPARC overexpression may play an important role in the initiation and development of non-small cell lung cancer (NSCLC), whereas KLF4 inhibits this process. PMID: 23249717
  80. This analysis suggests a potential role of SPARC in determining prognosis and response to primary chemotherapy in early breast cancer. PMID: 23638089
  81. High secreted protein acidic and rich in cysteine induces endoplasmic reticulum stress leading to autophagy-mediated apoptosis in neuroblastoma. PMID: 23123816
  82. role of SPARC, MMP-2 and MMP-9 in intracranial aneurysms PMID: 23516489
  83. SPARC expression is higher in squamous cell carcinoma than in adenocarcinoma in non-small cell lung cancer. SPARC was a prognostic factor of overall survival but not disease-free survival. PMID: 23114088
  84. The interaction between SPARC and integrin beta1 results in suppression of cell proliferation in prostate cancer. PMID: 23532895
  85. MiR-145 regulates glioma cell migration by targeting CTGF which downregulates SPARC expression. PMID: 23390502
  86. SPARC overexpression increases IOP in perfused cadaveric human anterior segments resulting from a qualitative change the juxtacanalicular-extracellular matrix. PMID: 23599341
  87. These findings suggest that exercise stimulates SPARC secretion from muscle tissues and that SPARC inhibits colon tumorigenesis by increasing apoptosis. PMID: 22851666
  88. SPARC is related to the invasive phenotype of cervical cancer cells. Knockdown of SPARC significantly suppresses cervical cancer cell proliferation, induces cell apoptosis and inhibits cell invasion and metastasis. PMID: 23050783
  89. Low endogenous SPARC expression is a constant feature of BCR/ABL positive cells and that imatinib treatment induces SPARC overproduction. PMID: 23383963
  90. Expression of miR-29a is important in the regulation of the SPARC gene expression and hepatocellular carcinoma growth. PMID: 23023935
  91. Conditioned medium from SPARC-overexpressed neuroblastoma cells (pSPARC-CM) suppressed expression of growth factors was mediated by inhibition of the Notch signaling pathway. PMID: 22956186
  92. SPARC is produced by benign and malignant compartments of bladder carcinomas where it functions to suppress bladder carcinogenesis, progression, and metastasis. PMID: 23321672
  93. IRE1alpha is a key regulator of SPARC expression in vitro in a glioma model. PMID: 22718352
  94. autocrine SPARC maintains heightened SLUG expression in melanoma cells and indicate that SPARC may promote EMT-associated tumor invasion by supporting AKT-dependent upregulation of SLUG. PMID: 22911700
  95. SPARC suppresses angiogenesis of gastric cancer by down-regulating the expression of VEGF and MMP-7 PMID: 22957090
  96. SPARC modulates the expression of several extracellular matrix genes in cultured human trabecular meshwork cells. PMID: 22136411
  97. SPARC is overexpressed in highly invasive subclone and ovarian cancer tissues and plays an important role in ovarian cancer growth, apoptosis and metastasis. PMID: 22879971
  98. The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human. PMID: 22880013
  99. Stromal SPARC contributes to the detrimental fibrotic changes associated with myeloproliferation whereas its deficiency favors myeloid cell expansion. PMID: 22955913
  100. SPARC promotes the development of erythroid progenitors, but does not affect terminal erythroid differentiation. PMID: 22687753

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Involvement in disease Osteogenesis imperfecta 17 (OI17)
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Protein Families SPARC family
Database Links

HGNC: 11219

OMIM: 182120

KEGG: hsa:6678

STRING: 9606.ENSP00000231061

UniGene: Hs.111779

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