Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/m.
Alternative Names
Scgb1a1; Cc10; Ugb; Utg; Uteroglobin; Clara cell 17 kDa protein; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1
Species
Mus musculus (Mouse)
Expression Region
22-96aa
Complete Sequence
DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF
Protein Length
Full Length of Mature Protein
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Advance your immunology research with Recombinant Mouse Uteroglobin (Scgb1a1), a multifunctional secretory protein with immunosuppressive and anti-inflammatory properties. This full-length, mature protein is expressed in E. coli and encompasses the 22-96aa region. With a tag-free format, a purity of over 98% as confirmed by SDS-PAGE and HPLC, and endotoxin levels below 1.0 EU/µg, this lyophilized powder is perfect for a wide range of research applications. Scgb1a1 demonstrates full biological activity, with an ED50 of less than 5.0 μg/ml as determined by its ability to support A549 human lung carcinoma cell adhesion, reflecting a specific activity of more than 200 IU/mg.
