Product Details

Purity

≥98% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/m.

Target Names

Scgb1a1

Uniprot No.

Q06318

Research Area

Immunology

Alternative Names

Scgb1a1; Cc10; Ugb; Utg; Uteroglobin; Clara cell 17 kDa protein; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

22-96aa

Complete Sequence

DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF

Mol. Weight

8.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Uteroglobin (Scgb1a1) plays a central role in modulating inflammation and immune responses in pulmonary tissue, making it a valuable tool for studying lung epithelial biology. This tag-free recombinant mouse protein, covering residues 22–96 of the mature sequence, demonstrates robust cell adhesion activity with an ED50 below 5.0 μg/ml against A549 human lung carcinoma cells, corresponding to a specific activity exceeding 200 IU/mg—evidence that supports use in functional cell adhesion assays, immune modulation studies, and as a quantitative standard in binding experiments. Expressed in *E. coli* to yield a properly folded homodimeric secretoglobin without glycosylation interference, this preparation achieves greater than 98% purity by SDS-PAGE and endotoxin levels below 1.0 EU/μg, satisfying the quality thresholds commonly required for cell-based functional assays and *in vivo* inflammation models where endotoxin contamination would confound results.

Target Background

Function

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

Gene References into Functions

the present results demonstrate that rCC16 has therapeutic effects on COPD by downregulating proinflammatory factors via the NFkappaB pathway. PMID: 29956762
This study extends the function of the Scgb1a1-expressing epithelial cell population as a major mediator of the antiviral response. It PMID: 29593031
Chronic P. aeruginosa inflammation resulted in chronic bronchitis and emphysematous changes in CCSP-deficient mice. PMID: 27703342
CC16 protects lungs from cigarette smoke (CS)-induced injury by reducing lung NF-kappaB activation. CS-induced airway CC16 deficiency increases CS-induced pulmonary inflammation and injury and likely contributes to the pathogenesis of COPD. PMID: 25700379
Depletion of bone marrow CCSP-expressing cells delays airway regeneration. PMID: 25409745
Serum CC-16 is associated with disease progression in chronic obstructive pulmonary disease (COPD). However, the absence of CC-16 does not appear to modify the risk of cigarette-related COPD in mice. PMID: 24245748
These findings indicate that CCSP has a regulatory role in obliterative bronchiolitis. PMID: 23997179
the role of clara cells and clara cell secretory protein in lung radiation injury exacerbated by viral infection PMID: 23621375
AAV2/9-CC10 vector virus guaranteed sufficient CC10 expression and had an anti-inflammatory effect in asthmatic mice. PMID: 23013277
Claudin-3 and Clara cell 10 kDa protein were identified as possible markers of early tobacco smoke-induced epithelial injury along alveolar ducts. PMID: 22659244
Scgb1a1-expressing cells, most likely Clara cells, are a major cell type that gives rise to alveolar type I and II cells during the regeneration of alveolar epithelia in response to severe pulmonary damage in mice. PMID: 23119022
These results indicate that CC10 gene transfer may inhibit airway inflammation through suppressing the activation of NF-kappaB. PMID: 22558282
Clara cell secretory protein expression is elevtaed in lungs after long-term n-hexane inhalation exposure. PMID: 20853679
CCSP promoter has a role in alveolar development PMID: 20693404
The expression of CC10 is downregulated in sinonasal mucosa in bacterial chronic rhinosinusitis. PMID: 19119605
Expression of SCGB1A1 mRNA was not decreased in vivo in the absence of C/EBPs. PMID: 21224212
The differential expression of mRNA by both airway level and lung region was determined for Clara cell secretory protein. PMID: 20852037
Lack of uteroglobin in mice enhances colonization of B16F10 melanoma cells in the lungs. PMID: 20118237
macrophages from Clara cell-depleted and CCSP(-/-) mice displayed increased Toll-like receptor 4 surface expression PMID: 19423773
Uteroglobin promoter-targeted c-MYC expression in transgenic mice cause hyperplasia of Clara cells and malignant transformation of T-lymphoblasts and tubular epithelial cells PMID: 11817538
Data show that Clara cell secretory protein (CCSP) is required for the appearance of secretory granules and that functional changes to Clara cells that result from CCSP deficiency lead to alterations in the composition of epithelial lining fluid. PMID: 12151308
demonstrate synergistic transactivation by C/EBPalpha and Nkx2.1 in the regulation of the CCSP gene PMID: 12161423
An immunohistochemical analysis was made of the expression of this protein in a transgenic model of mouse lung carcinogenesis. PMID: 12699910
Restoration of CCSP in the airways of CCSP-deficient mice abrogates the increase in viral persistence, lung inflammation, and airway hyperreactivity induced by respiratory syncytial virus infection. PMID: 12847279
Multiple mechanisms for repression of the Clara cell secretory protein gene during hyperoxia. PMID: 14500549
Data show that 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone exposure of Clara cell 10-kDa protein knock-out mice causes a significantly higher incidence of airway epithelial hyperplasia and lung adenomas compared with wild type littermates. PMID: 15148323
plays a direct role in the regulation of T-cell-mediated inflammatory responses. PMID: 15356574
We propose that mechanical activation of the cPLA2 pathway contributes to acute high PIP-induced lung injury and that CCSP may reduce this injury through inhibition of the cPLA2 pathway and reduction of proinflammatory products produced by this pathway. PMID: 15608088
Uteroglobin suppresses SCCA gene expression associated with airway inflammation in asthma PMID: 15677460
uteroglobin plays important roles in maintaining homeostasis in organs that are vulnerable to inadvertent stimulation of FP-mediated inflammatory response by inhibiting the prostaglandin F2alpha receptor PMID: 16061484
A critical role for uteroglobin in preventing the development of pulmonary fibrosis. PMID: 16872605
These results suggest that both long- and short-range paracrine signaling between nonciliated secretory cells and cells of the immune system and epithelium impact modification of cell type-specific proteins. PMID: 17384087
Treatment of lung neoplasms with bexarotene resulted in decreased mRNA expression. PMID: 17849452
Clara cells accumulated Clara cell secretory protein (CCSP) in Munc13-2-deficient mice. PMID: 18258655
preferentially expressed in mouse model of allergen-induced airway inflammation and remodeling PMID: 19322781

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Subcellular Location

Secreted.

Protein Families

Secretoglobin family

Tissue Specificity

Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).

Database Links

KEGG: mmu:22287

STRING: 10090.ENSMUSP00000025554

UniGene: Mm.2258

Code	CSB-AP000561MO
Abbreviation	Recombinant Mouse Scgb1a1 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse Uteroglobin protein (Scgb1a1) (Active)

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Target Background

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