ATG2B Antibody

Datasheet

Code	CSB-PA822186LA01HU
Size	US$166
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Image	Immunofluorescence staining of HepG2 cells with CSB-PA822186LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA822186LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATG2B Polyclonal antibody

Uniprot No.

Q96BY7

Target Names

ATG2B

Alternative Names

2410024A21Rik antibody; AI047755 antibody; AI503411 antibody; ATG2 autophagy related 2 homolog B antibody; Atg2b antibody; ATG2B_HUMAN antibody; Autophagy-related protein 2 homolog B antibody; AW558123 antibody; C030004M05Rik antibody; C14orf103 antibody; C630028L02Rik antibody; FLJ10242 antibody; KIAA4067 antibody; mKIAA4067 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Autophagy-related protein 2 homolog B protein (1357-1536AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATG2B Antibody (Product code: CSB-PA822186LA01HU) is Non-conjugated. For ATG2B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA822186LB01HU	ATG2B Antibody, HRP conjugated	ELISA
FITC	CSB-PA822186LC01HU	ATG2B Antibody, FITC conjugated
Biotin	CSB-PA822186LD01HU	ATG2B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Lipid transfer protein required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion. Lipid transfer activity is enhanced by WDR45/WIPI4, which promotes ATG2B-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes.

Gene References into Functions

Authors demonstrate that inhibition of autophagosome completion by Atg2A/B deletion accumulates immature autophagosomal membranes that promote non-canonical caspase-8 activation in response to nutrient starvation via an intracellular death-inducing signaling complex (iDISC). PMID: 28800131
This study highlights the transcriptional inactivation mechanisms of ATG2B, ATG4D, ATG9A and ATG9B promoter methylation status and the possible origin of autophagy signal pathway repression in invasive ductal carcinomas. PMID: 27265029
demonstrate that overexpression of ATG2B and GSKIP enhances hematopoietic progenitor differentiation, including of megakaryocytes, by increasing progenitor sensitivity to thrombopoietin PMID: 26280900
miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells. PMID: 25322940
Depletion of both Atg2A and Atg2B causes clustering of enlarged lipid droplets in an autophagy-independent manner. These data suggest that mammalian Atg2 proteins function both in autophagosome formation and regulation of lipid droplet morphology and dispersion. PMID: 22219374
miR-130a inhibited autophagy by reducing autophagosome formation, an effect mediated by downregulation of the genes ATG2B and DICER1. PMID: 22350415

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Subcellular Location

Preautophagosomal structure membrane; Peripheral membrane protein. Lipid droplet. Endoplasmic reticulum membrane; Peripheral membrane protein.

Protein Families

ATG2 family

Database Links

HGNC: 20187

OMIM: 616226

KEGG: hsa:55102

STRING: 9606.ENSP00000353010

UniGene: Hs.168241

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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