KCNE3 Antibody

Datasheet

Code	CSB-PA22569A0Rb
Size	US$166
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Image	IHC image of CSB-PA22569A0Rb diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA22569A0Rb diluted at 1:200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA22569A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) KCNE3 Polyclonal antibody

Uniprot No.

Q9Y6H6

Target Names

KCNE3

Alternative Names

KCNE3 antibody; Potassium voltage-gated channel subfamily E member 3 antibody; MinK-related peptide 2 antibody; Minimum potassium ion channel-related peptide 2 antibody; Potassium channel subunit beta MiRP2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Potassium voltage-gated channel subfamily E member 3 protein (1-57AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The KCNE3 Antibody (Product code: CSB-PA22569A0Rb) is Non-conjugated. For KCNE3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA22569B0Rb	KCNE3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22569C0Rb	KCNE3 Antibody, FITC conjugated
Biotin	CSB-PA22569D0Rb	KCNE3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Associated with KCNC4/Kv3.4 is proposed to form the subthreshold voltage-gated potassium channel in skeletal muscle and to establish the resting membrane potential (RMP) in muscle cells. Associated with KCNQ1/KCLQT1 may form the intestinal cAMP-stimulated potassium channel involved in chloride secretion that produces a current with nearly instantaneous activation with a linear current-voltage relationship.

Gene References into Functions

Regulation of human cardiac potassium channels by full-length KCNE3 and KCNE4 has been reported. PMID: 27922120
Structural, computational, biochemical, and electrophysiological studies lead to an atomically explicit integrative structural model of the KCNE3-KCNQ1 complex that explains how KCNE3 induces the constitutive activation of KCNQ1 channel activity, a crucial component in K(+) recycling. PMID: 27626070
Reported here are previously undiscovered protein-coding regions in exon 1 of hKCNE3 and hKCNE4 that extend their encoded extracellular domains by 44 and 51 residues, which yields full-length proteins of 147 and 221 residues, respectively. PMID: 27162025
Based on current evidence from published studies, neither of the two variants from KCNE was significantly associated with the risk of Meniere's disease--{REVIEW} PMID: 26890422
Data show that voltage-gated potassium channel KCNE3 directly affects the S4 movement in potassium channel KCNQ1. PMID: 26668384
KCNQ1/KCNE3 channels make only a small contribution to basolateral conductance in normal colonic crypts, with increased channel activity in UC appearing insufficient to prevent colonic cell depolarization in this disease. PMID: 26718405
KCNE1 and KCNE3: The yin and yang of voltage-gated K(+) channel regulation PMID: 26410412
A KCNE3 T4A mutation was identified in a Japanese patient presenting Brugada-pattern ECG and neurally mediated syncope. PMID: 22987075
Allele frequencies are studied for 11 known variants of KCNE3 gene, of which two (F66F and R83H) are polymorphic but are not associated with chronic tinnitus. PMID: 21899751
The results of this study indicated that Kv7.5 contributes to the spatial regulation. PMID: 22190306
2 of the 8 MiRP2 extracellular domain acidic residues (D54 and D55) are important for KCNQ1-MiRP2 constitutive activation. PMID: 20040519
data show that SNPs in KCNE1 and KCNE3 are not associated with Meniere disease in Caucasians PMID: 20034061
The KCNE3 protein within the micellesis in monomeric form and acquires mainly alpha-helical conformation. PMID: 19961415
Ectopic expression of KCNE3 accelerates cardiac repolarization and abbreviates the QT interval. PMID: 11956246
The authors found MiRP2-R83H in 3 of 321 control subjects and in 5 unaffected related individuals. Provocation of an unaffected carrier with glucose or KCl did not induce weakness. PMID: 15037716
interaction of MiRP2-72 with KCNQ1-338; and MinK-59,58 with KCNQ1-339, 340 PMID: 16308347
The result indicates that 112G/A SNP in the KCNE1 gene and 198T/C SNP in the KCNE3 gene could determine an increased susceptibility to develop MD. PMID: 16374062
The characterization of a missense mutation in MiRP2 that affects its phosphorylation and consequent interactions with Kv3.4 is reported. PMID: 16449802
KCNE3 also inhibits currents generated by Kv4.3 in complex with the accessory subunit KChIP2 PMID: 16782062
Up-regulation and incrased activity of KV3.4 channels and their accessory subunit Mirp2 induced by amyloid peptide are involved in apoptotic neuronal death. PMID: 17495071
Abnormalities in the KCNE3 gene is a potential genetic risk factor for initiation and/or maintenance of atrial fibrillation. PMID: 18209471
KCNE3 plays a functional role in the modulation of I(to) in the human heart and suggest that mutations in KCNE3 can underlie the development of BrS. PMID: 19122847

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasm. Perikaryon. Cell projection, dendrite. Membrane raft.

Protein Families

Potassium channel KCNE family

Tissue Specificity

Expressed in hippocampal neurons (at protein level). Widely expressed with highest levels in kidney and moderate levels in small intestine.

Database Links

HGNC: 6243

OMIM: 604433

KEGG: hsa:10008

STRING: 9606.ENSP00000310557

UniGene: Hs.523899

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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