Phospho-PIKFYVE (S307) Antibody

Code CSB-PA008031
Size US$167
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Product Details

Uniprot No. Q9Y2I7
Target Names PIKFYVE
Alternative Names 1 phosphatidylinositol 3 phosphate 5 kinase antibody; 1 phosphatidylinositol 4 phosphate 5 kinase antibody; 1-phosphatidylinositol 3-phosphate 5-kinase antibody; CFD antibody; epididymis luminal protein 37 antibody; FAB1 antibody; FAB1; S. cerevisiae; homolog of antibody; FYV1_HUMAN antibody; FYVE finger containing phosphoinositide kinase antibody; FYVE finger-containing phosphoinositide kinase antibody; KIAA0981 antibody; MGC40423 antibody; p235 antibody; Phosphatidylinositol 3 phosphate 5 kinase type III antibody; Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody; Phosphatidylinositol 3-phosphate 5-kinase antibody; Phosphatidylinositol 3-phosphate 5-kinase type III antibody; Phosphatidylinositol 4 phosphate 5 kinase type III antibody; Phosphoinositide kinase; FYVE finger containing antibody; PIKfyve antibody; PIP5K antibody; PIP5K3 antibody; PIPkin III antibody; PIPkin-III antibody; PtdIns(4)P 5 kinase antibody; Type III PIP kinase antibody; ZFYVE29 antibody; Zinc finger; FYVE domain containing 29 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human PIP5KIII around the phosphorylation site of S307.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Protocols Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression. The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and downregulates lipid product formation. Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport. Required for the maturation of early into late endosomes, phagosomes and lysosomes. Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network. Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets. In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes. Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS. Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome. Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation. Supports microtubule-based endosome-to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK. Mediates EGFR trafficking to the nucleus.; (Microbial infection) Required for cell entry of coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus EMC (HCoV-EMC) by endocytosis.
Gene References into Functions
  1. this study shows that PIKfyve coordinates the neutrophil immune response through the activation of the Rac GTPase PMID: 28779020
  2. in PC-3 cells inhibition of PIKfyve by apilimod or depletion by siRNA increased the secretion of the exosomal fraction. PMID: 27438886
  3. A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity. PMID: 26934981
  4. A novel heterozygous frameshift mutation (c.3151dupA) and a copy number variations in PIKFYVE gene have been found in two unrelated Fleck corneal dystrophy patients. PMID: 26396486
  5. The PIKfyve complex is required for APP trafficking, suggesting a feedback loop in which APP, by binding to and stimulating phosphatidylinositol-3,5-bisphosphate vesicle formation may control its own trafficking. PMID: 26216398
  6. APP functionally cooperates with PIKfyve in vivo. This regulation is required for maintaining endosomal and neuronal function. PMID: 26125944
  7. data identify a novel role of the ArPIKfyve-Sac3 complex in the mechanisms controlling aggregate formation of Sph1 and suggest that Sac3 protein deficiency or overproduction may facilitate aggregation of aggregation-prone proteins PMID: 26405034
  8. Data suggest PIKFYVE, MTMR3 (myotubularin related protein 3) and their product phosphatidylinositol 5-phosphate are involved in activation of RAC1 (rho family small GTP binding protein); this process regulates migration/invasion of carcinoma/sarcoma. PMID: 24840251
  9. Data indicate that pharmacological or genetic inactivation of PIKfyve rapidly induces expression of the transcription repressor ATF3, which is necessary and sufficient for suppression of type I IFN expression. PMID: 24600036
  10. Data indicate that AKT promotes EGFR recycling by phosphorylating and activating PIKfyve. PMID: 23757022
  11. the present observations show that PKB in conjunction with PIKfyve activates Kir2.1 channels. PMID: 23188060
  12. Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. PMID: 23154468
  13. The results provide the first experimental evidence that the principal pathway for PtdIns5P intracellular production is through PIKfyve. PMID: 22621786
  14. A novel c.3060-3063 delCCTT (p.P968Vfs23) mutation in the PIKFYVE gene has been described in a five generation Greek family, which segregated with the fleck corneal dystrophy. PMID: 22065932
  15. The recent advances in Arf6/PIP5K signaling and its linkage to cellular functions, are reviewed. PMID: 20945365
  16. The first multicellular model for PIKFYVE loss, pointing to a role in lysosome maturation PMID: 16801682
  17. REVIEW : PIKFYVE and other phosphoinositides regulatory proteins are implicated in human genetic diseases PMID: 18429927
  18. A phylogenetic study revealing co-evolution of phosphoinositides kinases and phosphatases ; PIKFYVE is absent from several organisms and co-evolved with VAC14 and FIG4 PMID: 18774718
  19. Coexpression of PIKfyve is followed by a marked increase of glutamate induced currents in EAAT2 expressing oocytes. PMID: 19910676
  20. p40 interaction with PIKfyve p40 os demonstrated; PIKfyve interaction and the subsequent PIKfyve-catalyzed p40 phosphorylation anchor p40 to discrete membranes facilitating late endosome-to-TGN transport. PMID: 14530284
  21. PIKfyve selectively regulates the sorting and traffic of peripheral endosomes containing lysosomaly directed fluid phase cargo through controlling the morphogenesis and function of multivesicular bodies PMID: 14551253
  22. Linkage analysis localized CFD to a 24-cM (18-Mb) interval of chromosome 2q35 flanked by D2S2289 and D2S126 and containing PIP5K3. PMID: 15902656
  23. PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs PMID: 16448788
  24. PIKfyve regulates endosome-to-TGN retrograde transport. PMID: 16954148
  25. results suggest that the local production of PtdIns(3)P implicates the fusion of macropinosomes via EEA1 as well as conventional early endosomes PMID: 17146146
  26. The observations disclose that PIKfyve participates in the SGK1-dependent regulation of SLC5A1. PMID: 17570343
  27. These data suggest for the first time a role of PtdIns5P and PIKfyve in oncogenesis, potentially linking intracellular trafficking to cancer. PMID: 18501703
  28. Kinesin adapter JLP links PIKfyve to microtubule-based endosome-to-trans-Golgi network traffic of furin. PMID: 19056739
  29. PIKfyve is a potent stimulator of ClC-2-activity and contributes to SGK1-dependent regulation of ClC-2. PMID: 19232516
  30. Results suggest that PIKfyve inhibition may render the late endosome/lysosome compartment refractory to fusion with both autophagosomes and with EGFR-containing multivesicular bodies. PMID: 19582903
  31. PIKfyve-dependent channel degradation is essential to prevent Ca2+-induced toxicity in neurons. PMID: 19841139
  32. these results demonstrate that PIKfyve regulates CFTR activity, and suggest a novel mechanism of CFTR regulation. PMID: 19852935

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Involvement in disease Corneal dystrophy, fleck (CFD)
Subcellular Location Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein.
Database Links

HGNC: 23785

OMIM: 121850

KEGG: hsa:200576

STRING: 9606.ENSP00000264380

UniGene: Hs.744997

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