SHPRH Antibody

Datasheet

Code	CSB-PA619905LA01HU
Size	US$166
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Image	IHC image of CSB-PA619905LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA619905LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SHPRH Polyclonal antibody

Uniprot No.

Q149N8

Target Names

SHPRH

Alternative Names

bA545I5.2 antibody; E3 ubiquitin-protein ligase SHPRH antibody; FLJ27258 antibody; FLJ37625 antibody; FLJ45012 antibody; FLJ90837 antibody; histone-linker antibody; KIAA2023 antibody; MGC134886 antibody; PHD and RING finger domain-containing helicase antibody; Shprh antibody; SHPRH_HUMAN antibody; SNF2 antibody; SNF2 histone linker PHD RING helicase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 ubiquitin-protein ligase SHPRH protein (1362-1512AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SHPRH Antibody (Product code: CSB-PA619905LA01HU) is Non-conjugated. For SHPRH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA619905LB01HU	SHPRH Antibody, HRP conjugated	ELISA
FITC	CSB-PA619905LC01HU	SHPRH Antibody, FITC conjugated
Biotin	CSB-PA619905LD01HU	SHPRH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.

Gene References into Functions

This study suggests that SHPRH could be a link between mTOR-mediated epigenetic regulations and rRNA transcription, while concomitantly affecting genomic integrity. PMID: 29139335
evidence that SHPRH functions in rRNA transcription through its interaction with histone H3 in a mammalian target of rapamycin (mTOR)-dependent manner PMID: 28400511
HLTF and SHPRH suppress mutagenesis in a damage-specific manner, preventing mutations induced by UV rays and methyl methanesulfonate. PMID: 21396873
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
SHPRH maps to 6q24 and is a possible candidate for the tumor suppressor gene reported from that region. PMID: 12837266
SHPRH function is an important deterrent to mutagenesis and carcinogenesis in humans PMID: 17108083
We report that a putative tumor suppressor gene, SHPRH, is a human orthologue of yeast RAD5.The yRad5/SHPRH-dependent pathway is a conserved and fundamental DNA repair mechanism that protects the genome from genotoxic stress. PMID: 17130289
HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. PMID: 18719106

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Protein Families

SNF2/RAD54 helicase family

Tissue Specificity

Broadly expressed.

Database Links

HGNC: 19336

OMIM: 608048

KEGG: hsa:257218

STRING: 9606.ENSP00000275233

UniGene: Hs.723297

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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