Recombinant Staphylococcus haemolyticus Dihydropteroate synthase(folP)

Code CSB-EP682423SLF
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The Latest Promotion
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names folP
Uniprot No. Q59919
Research Area others
Alternative Names folP; Dihydropteroate synthase; DHPS; EC 2.5.1.15; Dihydropteroate pyrophosphorylase
Species Staphylococcus haemolyticus
Source E.coli
Expression Region 1-267aa
Target Protein Sequence MTKTKIIGILNVTPDSFSDGGKYNSVDKAIARAKEMIDEGVDIIDVGGVSTRPGHTEVSLEEEMERVVPVVEQLVKLDVQISVDTYRSEVAEACLKLGATMINDQWAGLYDPKIFDVVSDYNAEIVLMHNGDGQREQPVVEEMLLSLLTQANKAEMAGIEKGNIWLDPGIGFAKSRSEEKEVMARLDELVATEYPVLLATSRKRFIKEMIGKETTPAERDEATAATTVYGIMKGIQAVRVHNVDLNVKLAQSIDFLKENEHERHHLS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 33.6 kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Q&A and Customer Reviews

 Q&A
Q:

I recently received their protein CSB-EP682423SLF (batch 03460), and was wondering about the buffer it is in. I am going to be making working aliquots of their protein, and I was wondering if they could receive either an extra vial of the buffer by itself, or if you could recommend a recipe for making more of the buffer themselves. Would you be able to provide either the extra vial, or the recipe?

A:
Thanks for your inquiry.We have listed the buffer component and concentration in the COA: Storage Buffer (20 mM Tris-HCl, 0.5 M NaCl, pH8.0, 20% glycerol). The concentration here refers to the molar concentration. You could calculate and prepare the buffer according to this recipe.
If the you prefer, we could also send extra 10ml of this buffer free of charge. It will take 3-7 working days.
Q:

I have 1M Tris-HCl in their lab, would you recommend diluting that with deionized H2O to get to the appropriate concentration?

A:
Thanks for your inquiry. You could add 200ul of 1M Tris-HCl, weigh 0.29g of NaCl, add deionized water to adjust PH, and finally volume to 10ml, and then you would get the corresponding buffer we provide.

Target Data

Function Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.
Protein Families DHPS family

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1