Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
lukF; Leukocidin-F subunit; Gamma-hemolysin; H-gamma-I subunit
Species
Staphylococcus aureus
Expression Region
26-323aa
Target Protein Sequence
AEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNAVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTLSRNTNYKNVGWGVEAHKIMNGWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDRAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 26-323 constitute the expression domain of recombinant Staphylococcus aureus lukF. This lukF protein is expected to have a theoretical molecular weight of 54 kDa. Expression of this lukF protein is conducted in e.coli. The N-terminal 10xHis-SUMO tag and C-terminal Myc tag was smoothly integrated into the coding gene of lukF, which enables a simple process of detecting and purifying the lukF recombinant protein in the following steps.
The Staphylococcus aureus leukocidin-F subunit (lukF) is one of the two subunits that make up the LukF-PV (Panton-Valentine leukocidin), a specific leukocidin associated with severe Staphylococcus aureus infections, including skin and soft tissue infections. The LukF-PV toxin is thought to form pores in the membranes of host immune cells, particularly white blood cells, disrupting their integrity and leading to cell death. This activity aids Staphylococcus aureus in evading the immune system and establishing infection. Understanding the structure and function of LukF, along with other leukocidins, is crucial for developing strategies to combat Staphylococcus aureus infections and addressing the associated pathogenicity.
