Recombinant Arabidopsis thaliana Flavonol synthase/flavanone 3-hydroxylase(FLS1)

In Stock
Code CSB-EP842601DOA
Size $2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names FLS1
Uniprot No. Q96330
Research Area others
Alternative Names
FLS1; FLS; At5g08640; MAH20.20Flavonol synthase/flavanone 3-hydroxylase; EC; EC; FLS 1
Species Arabidopsis thaliana (Mouse-ear cress)
Source E.coli
Expression Region 1-336aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 43.3 kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Could you please send me some informations to the following article # CSB-EP842601DOA and give me a recommendation in how to dilute this product (in which solution?)

Thanks for your inquiry.
Recombinant Arabidopsis thaliana Flavonol synthase/flavanone 3-hydroxylase(FLS1)
CSB-EP842601DOA >> E.coli
Express region:1-336aa;Full length protein.
Tag information:N terminal 10xHis-tagged + C terminal Myc-tagged


Buffier: 20 mM Tris-HCl, 0.5M NaCl, pH 8.0

Target Background

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin. In vitro catalyzes the oxidation of both enantiomers of naringenin to give both cis- and trans-dihydrokaempferol.
Gene References into Functions
  1. double mutant plants that harbor fls1kknock out in the pap1-D background (i.e., pap1-D/fls1ko plants) were generated, to examine whether anthocyanins can be further enhanced by blocking flavonol biosynthesis under PAP1 overexpression. PMID: 27562381
  2. Overexpression of FLS1 (FLS1-OX) not only altered seed coat color (resulting in a light brown color), but also affected flavonoid accumulation. Whereas fls1-3 mutants accumulated higher anthocyanin levels, FLS1-OX seedlings had lower levels than those of the wild-type. PMID: 26990404
Subcellular Location Cytoplasm. Nucleus.
Protein Families Iron/ascorbate-dependent oxidoreductase family
Tissue Specificity Expressed in young seedlings (at protein level). Expressed in roots, emerging leaves, shoot-root transition zone, trichomes, flowers and siliques. In cotyledons, expressed mostly on the adaxial side and only in guard cells on the abaxial side.
Database Links

KEGG: ath:AT5G08640

STRING: 3702.AT5G08640.1

UniGene: At.8771

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