Recombinant Arabidopsis thaliana F-box protein ORE9 (ORE9), partial

Code CSB-YP874428DOA
MSDS
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Source Yeast
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Code CSB-EP874428DOA
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Source E.coli
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Code CSB-EP874428DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874428DOA
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Source Baculovirus
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Code CSB-MP874428DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAX2
Uniprot No.
Alternative Names
MAX2; FBL7; KAI1; ORE9; At2g42620; F14N22.11; F-box protein MAX2; F-box/LRR-repeat protein 7; Protein KARRIKIN INSENSITIVE 1; Protein MORE AXILLARY BRANCHING 2; Protein ORESARA 9
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the senescence. Is necessary for responses to strigolactones and karrikins. Contributes to the selective repression of axillary shoots and moderates the branching by regulating negatively the auxin transport in primary stems, in an AXR1-independent manner. Required for the progression of leaf senescence mediated by methyl jasmonate. Required at each node to suppress axillary bud growth.
Gene References into Functions
  1. Stromule frequency correlates with strigolactone levels in the wild type and strigolactone mutants (max2-1 max3-9). PMID: 27760882
  2. Study revealed a clear MAX2-dependent rac-GR24 response: an increase in abundance of enzymes involved in flavonol biosynthesis, which was reduced in the max2-1 mutant. These results identified a large set of proteins shown to be regulated by the MAX2 function. PMID: 27317401
  3. This study provides genetic evidence that the functions of the MAX2 orthologs, and perhaps the MAX2 signaling pathways, are conserved in parasitic and non-parasitic plants. OaMAX2 of Orobanche aegyptiaca and Arabidopsis AtMAX2 share conserved functions in both development and drought responses. PMID: 27425246
  4. MAX2 signaling in the endodermis and/or quiescent center is partially sufficient to exert changes in F-actin density and cellular trafficking in the epidermis, and alter gene expression under low phosphate conditions. PMID: 26919985
  5. SMAX1 and SMXL6,7,8 repress karrikin and strigolactone signaling, respectively, and suggest that all MAX2-dependent growth effects are mediated by degradation of SMAX1/SMXL proteins. PMID: 26546447
  6. KAI2 degradation is independent of MAX2 and is not proteasome mediated. PMID: 25698586
  7. max2 mutant plants showed increased susceptibility to the bacterial necrotroph Pectobacterium carotovorum as well as to the hemi-biotroph Pseudomonas syringae but not to the fungal necrotroph Botrytis cinerea. PMID: 25849639
  8. An important role for MAX2 in plant responses to abiotic stress conditions, including regulation of drought tolerance PMID: 24198318
  9. FHY3 and MAX2 have opposite roles in both shoot branching and oxidative stress tolerance PMID: 22540368
  10. AGB1 and NDL1 positively regulate basipetal inflorescence auxin transport and modulate MAX2 expression in shoots. PMID: 24223735
  11. KAI2 and MAX2 define a regulatory pathway that largely operates independently of HY5 to mediate seedling responses to abiotic signals such as smoke and light. PMID: 23142794
  12. MAX2 mediated strigolactone signaling in roots. PMID: 23425316
  13. MAX2 modulates multiple hormone pathways to affect photomorphogenesis. PMID: 22466576
  14. a MAX2-dependent signal transduction mechanism was adapted to mediate responses to two chemical cues with distinct roles in plant ecology and development. PMID: 21555559
  15. Results show that axillary shoot growth is controlled locally, at the node, by an SCF(MAX2), the action of which is enhanced by the mobile MAX signal. PMID: 17346265
  16. roles in red, far-red, and ultraviolet-A/blue light-signaling pathways PMID: 17951458

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the vasculature of growing leaves and roots, rosette axillary bud, flowers, siliques, funiculi and stems.
Database Links

KEGG: ath:AT2G42620

STRING: 3702.AT2G42620.1

UniGene: At.21231

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