Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5(ABI5)

Code CSB-EP879765DOA
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ABI5
Uniprot No. Q9SJN0
Research Area Others
Alternative Names ABI5; BZIP39; DPBF1; GIA1; NEM1; At2g36270; F2H17.12Protein ABSCISIC ACID-INSENSITIVE 5; Dc3 promoter-binding factor 1; AtDPBF1; Protein GROWTH-INSENSITIVITY TO ABA 1; bZIP transcription factor 39; AtbZIP39
Species Arabidopsis thaliana (Mouse-ear cress)
Source E.coli
Expression Region 1-442aa
Target Protein Sequence MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 63.0kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.
Gene References into Functions
  1. SOM is reported to negatively regulate seeds germination by altering GA/ABA metabolism, here we found that AFP2 and ABI5 altered SOM transcription. Data demonstrate that AFP2 is a novel regulator to control high temperature-induced secondary seed dormancy through ABI5 and SOM. PMID: 29723526
  2. These data demonstrate that PHO1 is involved in ABA-mediated seed germination and early seedling development and transcriptionally regulated by ABI5. PMID: 29089393
  3. Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression PMID: 28438576
  4. ABI5 is a direct target gene of BZR1, and modulating the expression of ABI5 by BZR1 plays important roles in regulating the crosstalk between the brassinosteroid and abscisic acid signalling pathways. PMID: 27149247
  5. NF-YC9 mediates abscisic acid (ABA) signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. PMID: 28924726
  6. All of the study findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis. PMID: 28391398
  7. These results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination. PMID: 26564029
  8. DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. PMID: 26729600
  9. ABI5 bind to the ABR promoter, indicating that ABI5 directly regulates the expression of ABR. The disruption of ABI5 function in abr abi5-1 plants abolished the senescence-accelerating phenotype of the abr mutant, demonstrating that ABI5 is epistatic to ABR. PMID: 27095403
  10. RGL2 promotes the entrance in secondary dormancy through ABI5 action. PMID: 26177669
  11. link between nitric oxide and abscisic acid hormone signalling through distinct posttranslational modifications of ABI5 during early seedling development PMID: 26493030
  12. RAV1 plays an important role in abscisic acid signaling by modulating the expression of ABI3, ABI4, and AbI5 during seed germination and early seedling development. PMID: 25231920
  13. BIN2 phosphorylates and stabilizes ABI5 to mediate Abscisic acid (ABA) response during seed germination, while brassinosteroids (BRs) repress the BIN2-ABI5 cascade to antagonize ABA-mediated inhibition. PMID: 25415975
  14. ubiquitination promotes AB15 degradation; sumoylation prohibits degradation PMID: 24398698
  15. results suggest that ABA negatively regulates SHB1 expression, at least in part, through the action of its downstream signaling component ABI5 PMID: 24619610
  16. Transgenic cotton (Gossypium hirsutum) expressing AtRAV1/2 and/or AtABI5 showed resistance to imposed drought stress. PMID: 24483851
  17. ABI5 functions in the glucose-mediated inhibition of the root meristem zone by repressing PIN1 accumulation, thus leading to reduced auxin levels in roots. PMID: 24237322
  18. our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana. PMID: 24586210
  19. These results define a genetic pathway through which cytokinin specifically induces the degradation of ABI5 protein. PMID: 24443524
  20. Overexpression of TAP46 and inhibition of activities of PP2A or PP2A-like protein phosphatases can increase transcript levels of several ABI5-regulated genes. PMID: 24357600
  21. The function of AtSAG in abscisic acid (ABA)signalling depended on ABI3 and ABI5. AtSAG is an important negative regulator of ABA signalling during seed germination and seedling development. PMID: 24163287
  22. These data suggest that Ala214 in ABI5 contributes to the function of ABI5 via its interaction with ABI3. PMID: 23299338
  23. ABI5 phosphorylation is regulated by PP6 phosphatase. PMID: 23404889
  24. Data indicate that KEEP ON GOING (KEG) E3 interacts with abscisic acid insensitive 5 (ABI5) in the cytoplasm. PMID: 23720747
  25. The inhibitory effect of abscisic acid on floral transition is mediated by ABI5. PMID: 23307919
  26. MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. PMID: 22822206
  27. one-hybrid assays demonstrated synergistic action of ABI4 with ABI5 or related bZIP factors in regulating target promoters, and mutant analyses showed that ABI4 and these bZIPs share some functions PMID: 21243515
  28. Abscisic acid promotes ABI5 accumulation by inducing the ubiquitination and proteasomal degradation of KEG. PMID: 20682837
  29. The redundancy of function among genes in the families of ABF3 and ABI5 in A. thaliana is reported. PMID: 16247556
  30. Results show that over-expression of ABI5 rendered phas expression ABA-independent in the presence of PvALF. PMID: 18038114
  31. PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. PMID: 19255443
  32. SIZ1-dependent sumoylation of ABI5 attenuates abscisic acid signaling PMID: 19276109

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Subcellular Location Nucleus
Protein Families BZIP family, ABI5 subfamily
Tissue Specificity Predominantly expressed in seeds.
Database Links

KEGG: ath:AT2G36270

STRING: 3702.AT2G36270.1

UniGene: At.14114

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