Recombinant Arabidopsis thaliana Two pore calcium channel protein 1 (TPC1), partial

1. Structural basis for activation of voltage sensor domains in an ion channel TPC1. PMID: 30190435
2. variant (TPC1D454N) depolarizes endomembranes by increasing cation fluxes towards the cytosol PMID: 28885692
3. The ion selectivity of AtTPC1 was determined and compared with the selectivity of human TPC2. TPC1 is selective for Ca(2+) over Na(+), but nonselective among monovalent Li(+), Na(+), and K(+)). AtTPC1 could be converted to a Na(+)-selective channel by mimicking the selectivity filter of HsTPC2. Key residues were identified in the TPC filters that differentiate this selectivity. A Na+-selective mutant was characterized. PMID: 28096396
4. TPC1 role in the systemic response to salt stress PMID: 27261066
5. Structure-function analysis of the Arabidopsis TPC1 channel in planta confirmed that helix S10 operates as the major voltage-sensing site, with Glu450 and Glu478 identified as possible ion-pair partners for voltage-sensing Arg537. PMID: 27270880
6. The domain architecture of TPC1 has been discussed. PMID: 27156118
7. This study discovered that the dimerization of a predicted helix within the carboxyl-terminus is essential for the activity of TPC1. PMID: 26781468
8. crystal structure of TPC1 from Arabidopsis thaliana at 2.87 A resolution as a basis for understanding ion permeation, channel activation, the location of voltage-sensing domains and regulatory ion-binding sites PMID: 26961658
9. crystal structure of a vacuolar two-pore channel from Arabidopsis thaliana, AtTPC1, which functions as a homodimer PMID: 26689363
10. The SV channel fou2 mutation in TCP1 altered sensitivity to luminal Ca2+ levels. PMID: 19298454
11. TPC1 contains a N-terminal dileucine motif (EDPLI) critical for vacuolar targeting PMID: 22490017
12. TPC1 has a role in maintaining differential K and Ca storage across the leaf; it releases Ca2+ from epidermal and bundle sheath cell vacuoles to maintain low [Ca]vac. PMID: 22067997
13. EF-hand 1 modulates the Ca2+-sensitivity of TPC1, whereas EF-hand 2 is essential for the Ca2+-dependent channel opening. PMID: 21736651
14. Results indicate that Glu-450 and Asp-454 of the slow vacuolar channel TPC1 are directly involved in Ca2+ binding, whereas Glu-456 and Glu-457 are probably involved in connecting the luminal Ca2+ binding site to the channel gate. PMID: 21764990
15. AtTPC1 functions in response to external Ca2+ but not to abscisic acid and methyl jasmonate in Arabidopsis guard cells. PMID: 20061305
16. Results suggest that members of TPC1 channel family are reactive oxygen species-responsive calcium channels and are the possible targets of aluminum-dependent inhibition. PMID: 15464979
17. a tpc1 knockout mutant lacks functional slow vacuolar channel activity and is defective in both abscisic acid-induced repression of germination and in the response of stomata to extracellular calcium PMID: 15772667
18. a genetic screen capable of detecting the misregulated activity of 13-lipoxygenase identified a missense mutation in TPC1, indicating that cation fluxes exert control over positive feedback loop for jasmonic acid stimulates its own synthesis. PMID: 17253984
19. results suggest that wild-type TPC1 contributes to their expression of PDF1.2a and THI2 by mechanisms somewhat different from those affecting their expression in fou2 PMID: 17981874
20. study found loss of TPC1 did not change membrane hyperpolarization-activated Ca(2+)-permeable channel activity; we conclude TPC1, under physiological conditions, functions as a vacuolar cation channel without major impact on cytosolic Ca(2+) homeostasis PMID: 18028262

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KEGG: ath:AT4G03560

STRING: 3702.AT4G03560.1

UniGene: At.3957