Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial

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Code CSB-EP711569BAAB
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
htpG
Uniprot No.
Research Area
others
Alternative Names
htpG; BF2491; Chaperone protein HtpG; Heat shock protein HtpG; High temperature protein G
Species
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Source
E.coli
Expression Region
1-326aa
Target Protein Sequence
MQKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFLPVPIAFGKKKEWKDGKQVETAEDNVINDTIPLWTKKPSELSDEDYKKFYRELYPMSDEPLFWIHLNVDYPFHLTGILYFPKVKSNIDLNKNKIQLYCNQVYVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
57.0 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Recombinant B. fragilis htpG expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this htpG protein. The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The htpG protein expression in E.coli is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers.

ClpB and HtpG promote de novo protein folding in stressed E.coli cells. HtpG is a prokaryotic homolog of Hsp90 that stabilizes the bile body protein in the cyanobacterium Polychlorococcus elongatus PCC 7942. HtpG plays a role in cold acclimation in cyanobacteria. HtpG protein does not act as a cellular thermometer in Bacillus subtilis. The expression of htpG was found to be significantly up-regulated in P. plecoglossicida at its virulence temperature of 18°C.

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Target Background

Function
Molecular chaperone. Has ATPase activity.
Subcellular Location
Cytoplasm.
Protein Families
Heat shock protein 90 family
Database Links
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