Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

htpG

Uniprot No.

Q5LCH4

Research Area

others

Alternative Names

htpG; BF2491; Chaperone protein HtpG; Heat shock protein HtpG; High temperature protein G

Species

Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

Source

E.coli

Expression Region

1-326aa

Target Protein Sequence

MQKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFLPVPIAFGKKKEWKDGKQVETAEDNVINDTIPLWTKKPSELSDEDYKKFYRELYPMSDEPLFWIHLNVDYPFHLTGILYFPKVKSNIDLNKNKIQLYCNQVYVTDSVEGIVPDFLTLLHGVLDSPDIPLNVSR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

57.0 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant B. fragilis htpG expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this htpG protein. The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The htpG protein expression in E.coli is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers.

ClpB and HtpG promote de novo protein folding in stressed E.coli cells. HtpG is a prokaryotic homolog of Hsp90 that stabilizes the bile body protein in the cyanobacterium Polychlorococcus elongatus PCC 7942. HtpG plays a role in cold acclimation in cyanobacteria. HtpG protein does not act as a cellular thermometer in Bacillus subtilis. The expression of htpG was found to be significantly up-regulated in P. plecoglossicida at its virulence temperature of 18°C.

Target Background

Function

Molecular chaperone. Has ATPase activity.

Subcellular Location

Cytoplasm.

Protein Families

Heat shock protein 90 family

Database Links

KEGG: bfs:BF9343_2410

STRING: 272559.BF2491

Code	CSB-EP711569BAAB
Abbreviation	Recombinant Bacteroides fragilis htpG protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Bacteroides fragilis Chaperone protein htpG (htpG), partial

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