The full-length cDNA ORF of Calloselasma rhodostoma Snaclec rhodocytin subunit alpha was N-terminally tagged a 6xHis-SUMO and then expressed in the E.coli cells. The recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha thus obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, this protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant Snaclec rhodocytin subunit alpha protein may be used to produce specific antibodies or in the studies of snaclec rhodocytin-mediated signal transduction.
Snaclec rhodocytin is a disulfide‐linked heterodimeric C‐type lectin derived from Calloselasma rhodostoma and is made up of α‐subunits and β‐subunits. The interaction between snaclec rhodocytin and C‐type lectin‐like receptor 2 (CLEC-2) induces platelet aggregation. CLEC‐2 physiologically binding to podoplanin (PDPN), which is expressed on some tumor cell types, participates in tumor cell‐induced platelet aggregation and tumor metastasis. This may propose a possible source of anti‐CLEC‐2 drugs for both antiplatelet and antimetastasis therapy through rhodocytin modification.
||Greater than 90% as determined by SDS-PAGE.
||; Snaclec rhodocytin subunit alpha; Aggretin alpha chain; Rhodoaggretin subunit alpha
||Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.