Name: Recombinant Candida albicans Candidapepsin-9 (SAP9)
Brand: CUSABIO
SKU: CSB-YP522117CZF

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SAP9

Uniprot No.

O42779

Research Area

Neuroscience

Alternative Names

SAP9Candidapepsin-9; EC 3.4.23.24; ACP 9; Aspartate protease 9; Secreted aspartic protease 9

Species

Candida albicans (Yeast)

Source

Yeast

Expression Region

18-544aa

Target Protein Sequence

AKAPFKIDFEVRRGESKDDLSPEDDSNPRFVKRDGSLDMTLTNKQTFYMATLKIGSNEDENRVLEDTGSSDLWVMSHDLKCVSAPISKRNERSFGHGTGVKLNERELMQKRKNLYQPSRTIETDEEKEASEKIHNKLFGFGSIYSTVYITEGPGAYSTFSPLVGTEGGSGGSGGSNTCRSYGSFNTENSDTFKKNNTNDFEIQYADDTSAIGIWGYDDVTISNVTVKDLSFAIANETSSDVGVLGIGLPGLEVTTQLRYTYQNLPLKLKADGIIAKSLYSLYLNTADAKAGSILFGAIDHAKYQGDLVTVKMMRTYSQISYPVRIQVPVLKIDVESSSGSTTNILSGTTGVVLDTGSTLSYVFSDTLQSLGKALNGQYSNSVGAYVVNCNLADSSRTVDIEFGGNKTIKVPISDLVLQASKSTCILGVMQQSSSSSYMLFGDNILRSAYIVYDLDDYEVSLAQVSYTNKESIEVIGASGITNSSGSGTTSSSGTSTSTSTRHSAGSIISNPVYGLLLSLLISYYVLV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

58.9kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CUSABIO used yeast cells to express N-terminal 6xHis-tagged Candida albicans Candidapepsin-9 (SAP9). This recombinant SAP9 protein is full-length of mature protein containing 18-54aa of Candida albicans SAP9. It underwent validation via SDS-PAGE analysis. Its purity reached up to 90%. Under reducing conditions, a molecular weight band of around 60 kDa was visualized on the SDS-PAGE gel. This recombinant SAP9 protein may be applied to produce specific anti-SAP9 antibodies or in the studies of neuroscience.

Secreted aspartic protease 9 (SAP9) is a potential hyphal-associated factor responsive to serum hyphal-inducing stimuli through a cAMP-protein kinase A pathway mediated by EFG1. It plays a crucial role in serum induced-hyphal formation. And it is conducive to the process of invasion of Candida albicans into the epithelial cells, resulting in host cell damage. Besides, SAP9 is involved in protein–receptor interactions between fungal cells, and the formation of polymicrobial biofilm communities via interkingdom communication.

Code	CSB-YP522117CZF
Abbreviation	Recombinant Candida albicans SAP9 protein
MSDS	MSDS Datasheet
Size	$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Candida albicans Candidapepsin-9 (SAP9)

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