Recombinant Chicken Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (LITAF)

Code CSB-YP851221CH
MSDS
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Source Yeast
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Code CSB-EP851221CH
MSDS
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Source E.coli
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Code CSB-EP851221CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851221CH
MSDS
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Source Baculovirus
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Code CSB-MP851221CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LITAF
Uniprot No.
Alternative Names
LITAF; SIMPLE; Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; LPS-induced TNF-alpha factor homolog; Small integral membrane protein of lysosome/late endosome
Species
Gallus gallus (Chicken)
Expression Region
1-148
Target Protein Sequence
MSAPSGFPAP SAPPSYEETV GINVNYPHPY PVPQPGLRPD GKGMNPPQYS GQPMPTSTPV TVQTVYVQQP VVLFYDRPVQ MSCPSCNQMI VTRLCYESGA LTWLSCGGLF LLGCIAGCCL IPFCVDALKD VEHFCPNCNA HVGSYKRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role in the nucleus in regulating the expression of numerous cytokines.
Gene References into Functions
  1. The results showed that Se deficiency induced high expression levels of PTGE, COX-2, TNF-alpha, and NF-kappaB in the gastrointestinal tract tissues. PMID: 26899319
  2. The results showed that the expression levels of IL-6, IL-8, TNF-alpha, and NF-kappaB which seemed to be a critical mediator in the inflammatory response tended to increase in the birds chronically treated with As2O3. However, the mRNA expression levels of IL-4, IL-12beta, and IL-1beta were decreased in the experiment. PMID: 26276563
  3. Data show that IL-10, IFN-gamma and TNF-alpha contents in the cecal tonsil were significantly decreased in the 30, 45 and 60 mg/kg groups, while IL-6 content in the cecal tonsil was only decreased in 60 mg/kg at 14 and 28 days of age. PMID: 22270623
  4. The expression of interleukin(IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha in amyoidosis arthropathy in chickens was studied. PMID: 18612836
  5. IL1B and LITAF mRNAs are increased in the vagina during insemination. This response may lead to sperm degradation and elimination. PMID: 18539728

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Subcellular Location
Cytoplasm. Nucleus. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane. Late endosome membrane. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane.
Protein Families
CDIP1/LITAF family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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