Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Microbiology
Species
Chicken anemia virus (isolate USA 26p4) (CAV)
Expression Region
1-120aa
Target Protein Sequence
MNALQEDTPPGPSTVFRPPTSSRPLETPHCEIRIGIAGITITLSLCGCANARAPTLRSATADSSESTGFKNAPDLRTDQPKPPSKKRSCDPSEYRVSELKENLITTTPSRPRTARRCIRL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression vector recombined with the recombinant DNA was transformed into the E.coli for expression. The recombinant DNA resulted from the fusion of the gene coding for the 1-120aa of the chicken anemia virus VP3 protein and the N-terminal 6xHis tag gene. The product was purified and isolated to get the recombinant chicken anemia virus VP3 protein with N-terminal 6xHis tag. The purity of this recombinant VP3 protein reaches up to 90%. Under SDS-PAGE condition, this recombinant VP3 protein showed a band with a molecular weight of about 20 kDa on the gel. It may be used in the microbiology studies.
VP3 is a gene encoding a protein named chicken anemia virus Apoptin (abbreviated Apoptin) and belongs gyrovirus apoptin family. This protein is 121 amino acids in length and has a mass of 13.6 kDa and contains a bipartite C-terminal nuclear localization signal (NLS) from residues 82–88 and 111–121, which permits its translocation to the nucleus in a cell-type-specific manner. Apoptin infects the thymocytes and erythroblasts in young chickens, causing chicken infectious anemia and immunosuppression. Apoptin has subsequently been shown to induce apoptosis in many human cancer cell lines in a manner that is independent of p53. Apoptin's tumor-cell killing ability has generated significant interest for its potential in anticancer therapy, and indeed apoptin has been investigated extensively in a wide range of human tumor cell.
