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Recombinant Chlamydophila felis tRNA uridine (34) hydroxylase (trhO)

Recombinant Chlamydophila felis tRNA uridine (34) hydroxylase (trhO)

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Code	CSB-EP636612CAAV
Abbreviation	Recombinant Chlamydia felis trhO protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

trhO

Uniprot No.

Q252M0

Research Area

Others

Alternative Names

(34)(tRNA hydroxylation protein O)

Species

Chlamydia felis (strain Fe/C-56) (Chlamydophila felis)

Source

E.coli

Expression Region

1-325aa

Target Protein Sequence

MKKNYYALAYYHFTRVDNPQEEIALHKELFKKLDVSCRIYISEQGINGQFSGYQPDAEYYMNWLKQRPGFSNVKFKIHHIEENIFPRATVKYRKELVALGCDVDLSNQGKHISPKEWHEKLEENRCLVLDVRNNYEWKIGHFENAVLPDIQTFREFPEYAEQLSKEHDPETTPVMMYCTGGIRCELYSSLLLEKGFKEVYQLDGGVIAYGQAMGTGKWRGKLFVFDDRLAVPIDEADTDVSPIAQCSHCEASCDTYYNCANTDCNNLFICCEECIHSTKGCCSQECSQAPRIRSFSTSRGNKPFRRMHLCEISEEQEKPLSCCLR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

43.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Chlamydia felis (strain Fe/C-56) trhO protein production is accomplished by manipulating the expression of the desired gene in e.coli cells. The sequence (1-325aa) of foreign DNA is incorporated into an expression vector and subsequently introduced into e.coli cells. Positive cells are selected and cultured to induce the expression of the desired protein. The protein carries a N-terminal 6xHis tag. The resulting recombinant Chlamydia felis (strain Fe/C-56) trhO protein undergoes affinity purification, with a purity surpassing 90%, as measured by SDS-PAGE.