Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput (Clk), partial

1. experiments define the genetic architecture required to initiate circadian clock function in Drosophila, reveal mechanisms governing circadian activator stability that are conserved in perhaps all eukaryotes, and suggest that Clk, cyc, and cry expression is sufficient to drive clock expression in naive cells PMID: 28973907
2. propose that the dCLK/CYC-controlled TTFL operates differently in subsets of pacemaker neurons, which may contribute to their specific functions PMID: 27489346
3. ClkAR mutants showed significantly faster age-related locomotor deficits. Accelerated locomotor decline of the ClkAR mutant required expression of the PDF receptor and correlated to an apparent loss of dopaminergic neurons in the brain PMID: 28072817
4. these results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription. PMID: 25952406
5. Our findings suggest a novel role for clock protein phosphorylation in governing the relative strengths of entraining modalities by adjusting the dynamics of circadian gene expression. PMID: 25121504
6. These results demonstrate that CLK phosphorylation influences the circadian period by regulating CLK activity and progression through the feedback loop. PMID: 24872414
7. Computational dissection of CLK/CYC context-specific binding sites reveals sequence motifs for putative partner factors, which are predictive for individual binding sites PMID: 24332542
8. usp8 loss of function (RNAi) or expression of a dominant-negative form of the protein (USP8-DN) enhances CLK/CYC transcriptional activity and alters fly locomotor activity rhythms PMID: 23154984
9. CLK has specific targets in different tissues, implying that important CLK partner proteins and/or mechanisms contribute to gene-specific and tissue-specific regulation PMID: 22085964
10. dPER(DeltaCBD) does not provoke the daily hyperphosphorylation of dCLOCK, indicating that direct interactions between dPER and dCLOCK are necessary for the dCLOCK phosphorylation PMID: 20980603
11. findings show that Clk and cyc act during starvation to modulate the conflict of whether flies sleep or search for food PMID: 20541409
12. Here we examined the consequences of loss of clock function on reproductive fitness in Drosophila melanogaster with mutated period (per(0)), timeless (tim(0)), cycle (cyc(0)), and Clock (Clk(Jrk)) genes PMID: 11854509
13. dCLK levels are critical in mediating the direct photostimulation of locomotor activity in Drosophila. PMID: 11931742
14. constitutive levels of nuclear CLK regulate rhythmic transcription in circadian oscillator cells and CLK contributes to other behavioral processes by regulating gene expression in non-oscillator cells PMID: 16603674
15. findings suggest that Clockwork Orange (CWO) acts preferentially in the late night to help terminate CLK-CYC-mediated transcription of direct target genes including cwo itself PMID: 17578907
16. Cwo is rhythmically expressed and directly regulated by CLK-CYC through canonical E-box sequences. PMID: 17578908
17. Results indicate that, in vivo, clockwork orange (CWO) modulates clock gene expression through both repressor and activator transcriptional functions. PMID: 18375860
18. These results define the temporal and spatial coordinates of factors that initiate Clk expression, imply that circadian photoreceptors are not activated until the end of embryogenesis. PMID: 19094242
19. DBT plays a novel noncatalytic role in recruiting additional kinases that phosphorylate CLK, thereby repressing transcription PMID: 19139270
20. the post-translational processing of the circadian CLOCK protein by Cytoplasmic interaction with CYCLE protein PMID: 19376119
21. Sequential phosphorylation and subcellular distribution regulate the activity of the CLK protein. PMID: 19564332
22. Results reveal clear heterogeneity in Clock gene expression in the brain and provide a necessary focus to isolate novel transcription factors that bind at the Clk locus to regulate expression in different oscillator neuron subgroups. PMID: 19755581

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KEGG: dme:Dmel_CG7391

STRING: 7227.FBpp0099478

UniGene: Dm.7596