Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

phrB

Uniprot No.

P00914

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

phrB; phr; b0708; JW0698; Deoxyribodipyrimidine photo-lyase; EC 4.1.99.3; DNA photolyase; Photoreactivating enzyme

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-472aa

Target Protein Sequence

MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWATHNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNELIWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQPIVEHKEARVQTLAAYEAARKGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

73.7kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-472 form the expressed segment for recombinant Escherichia coli (strain K12) phrB. The calculated molecular weight for this phrB protein is 73.7 kDa. The phrB protein was expressed in e.coli. The N-terminal 10xHis-SUMO tag and C-terminal Myc tag was smoothly integrated into the coding gene of phrB, which enables a simple process of detecting and purifying the phrB recombinant protein in the following steps.

Deoxyribodipyrimidine photo-lyase (PhrB) in Escherichia coli is a crucial enzyme involved in the repair of DNA damage caused by ultraviolet (UV) radiation. UV radiation can induce the formation of cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts in DNA, which can lead to mutations and genomic instability. PhrB, a photolyase enzyme, specifically recognizes and repairs these UV-induced lesions. It utilizes visible light as an energy source to break the covalent bonds within the damaged DNA, thereby restoring the original structure of the nucleotides. The repair mechanism facilitated by PhrB is known as photoreactivation, and it contributes to the maintenance of genomic integrity in bacteria exposed to UV light. Studying PhrB provides insights into DNA repair processes and has implications for understanding the adaptive responses of bacteria to environmental stressors such as sunlight.

Target Background

Function

Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

Gene References into Functions

Data indicate that only the anionic hydroquinone flavin can be the functional state in DNA photolyase. PMID: 23882072
Data show that DNA photolyase exhibits a distinct reduction-potential gradient along the same aromatic residues with favorable reorganization energies to drive a highly unidirectional electron flow toward the active-site center from the protein surface. PMID: 23882080
Photoreduction by intraprotein electron transfer is not part of DNA photolyase photocycle under physiological conditions. PMID: 15568802
Fourier transform infrared (FT-IR) spectroscopy was used to monitor vibronic bands of the blue radical form and of the fully reduced FADH- form of the enzyme PMID: 15819881
We demonstrate that electron transfer does occur upon excitation of FADH degrees and leads to the formation of FADH- and a deprotonated tryptophanyl radical, most likely W359 degrees. PMID: 17696363

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Protein Families

DNA photolyase class-1 family

Database Links

KEGG: ecj:JW0698

STRING: 316385.ECDH10B_0775

Code	CSB-EP360595ENV
Abbreviation	Recombinant E.coli phrB protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360595ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) phrB. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360595ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) phrB.
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Recombinant Escherichia coli Deoxyribodipyrimidine photo-lyase (phrB)

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