Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
glpD; glyD; b3426; JW3389Aerobic glycerol-3-phosphate dehydrogenase; EC 1.1.5.3
Species
Escherichia coli (strain K12)
Expression Region
1-501aa
Target Protein Sequence
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-501 constitute the expression domain of recombinant Escherichia coli (strain K12) glpD. This glpD protein is theoretically predicted to have a molecular weight of 60.8 kDa. This protein is generated in a e.coli-based system. The N-terminal 6xHis tag was smoothly integrated into the coding gene of glpD, which enables a simple process of detecting and purifying the glpD recombinant protein in the following steps.
The Escherichia coli aerobic glycerol-3-phosphate dehydrogenase (GlpD) is an enzyme that plays a crucial role in glycerol metabolism. Specifically, GlpD catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate while transferring electrons to molecular oxygen. This process is part of the aerobic respiration pathway in E. coli, contributing to the generation of energy and metabolic intermediates. Research on GlpD in E. coli is significant for understanding bacterial energy metabolism and adaptation to different environmental conditions. The enzyme's involvement in glycerol utilization is particularly relevant for bacteria that encounter varying carbon sources, such as those transitioning between anaerobic and aerobic conditions. Investigating the structure and function of GlpD can provide insights into the regulation of glycerol metabolism and its importance in bacterial physiology.
