Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

glpD

Uniprot No.

P13035

Research Area

Others

Alternative Names

glpD; glyD; b3426; JW3389Aerobic glycerol-3-phosphate dehydrogenase; EC 1.1.5.3

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-501aa

Target Protein Sequence

METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

60.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-501 constitute the expression domain of recombinant Escherichia coli (strain K12) glpD. This glpD protein is theoretically predicted to have a molecular weight of 60.8 kDa. This protein is generated in a e.coli-based system. The N-terminal 6xHis tag was smoothly integrated into the coding gene of glpD, which enables a simple process of detecting and purifying the glpD recombinant protein in the following steps.

The Escherichia coli aerobic glycerol-3-phosphate dehydrogenase (GlpD) is an enzyme that plays a crucial role in glycerol metabolism. Specifically, GlpD catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate while transferring electrons to molecular oxygen. This process is part of the aerobic respiration pathway in E. coli, contributing to the generation of energy and metabolic intermediates. Research on GlpD in E. coli is significant for understanding bacterial energy metabolism and adaptation to different environmental conditions. The enzyme's involvement in glycerol utilization is particularly relevant for bacteria that encounter varying carbon sources, such as those transitioning between anaerobic and aerobic conditions. Investigating the structure and function of GlpD can provide insights into the regulation of glycerol metabolism and its importance in bacterial physiology.

Target Background

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.

Subcellular Location

Cytoplasm.

Protein Families

FAD-dependent glycerol-3-phosphate dehydrogenase family

Database Links

KEGG: ecj:JW3389

STRING: 316385.ECDH10B_3600

Code	CSB-EP320174ENV
Abbreviation	Recombinant E.coli glpD protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Aerobic glycerol-3-phosphate dehydrogenase (glpD)

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