Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
nrdD; b4238; JW4197; Anaerobic ribonucleoside-triphosphate reductase; EC 1.1.98.6; Class III ribonucleoside-triphosphate reductase
Species
Escherichia coli (strain K12)
Expression Region
1-712aa
Target Protein Sequence
MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAAVVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEIRGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIHYHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAIRDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWENENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLEGVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVYDNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPGVTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDVWDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQIG
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-712 form the expressed segment for recombinant Escherichia coli (strain K12) nrdD. This nrdD protein is expected to have a theoretical molecular weight of 84 kDa. Expression of this nrdD protein is conducted in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of nrdD, making it easier to detect and purify the nrdD recombinant protein in the later stages of expression and purification.
The Escherichia coli Anaerobic ribonucleoside-triphosphate reductase, encoded by the nrdD gene, is a key enzyme involved in nucleotide biosynthesis. Specifically, NrdD is a subunit of the anaerobic class III ribonucleotide reductase (RNR) complex. The RNR enzyme plays a crucial role in the reduction of ribonucleotides to deoxyribonucleotides, a fundamental step in DNA synthesis. Under anaerobic conditions, NrdD cooperates with other subunits in the RNR complex to catalyze the conversion of ribonucleoside triphosphates (NTPs) into deoxyribonucleoside triphosphates (dNTPs), providing the necessary building blocks for DNA replication and repair. Research on NrdD and anaerobic RNR contributes to the understanding of bacterial nucleotide metabolism and provides potential targets for antimicrobial drug development.