Recombinant Escherichia coli Heat-labile enterotoxin B chain (eltB)

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Code CSB-EP315643ENL
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
eltB
Uniprot No.
Research Area
Others
Alternative Names
eltB; ltpBHeat-labile enterotoxin B chain; LT-B; human; LTH-B
Species
Escherichia coli
Source
E.coli
Expression Region
22-124aa
Target Protein Sequence
APQSITELCSEYHNTQIYTINDKILSYTESMAGKREMVIITFKSGATFQVEVPGSQHIDSQKKAIERMKDTLRITYLTETKIDKLCVWNNKTPNSIAAISMEN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
17 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The expression region of this recombinant Escherichia coli eltB covers amino acids 22-124. This eltB protein is expected to have a theoretical molecular weight of 17 kDa. This eltB protein is produced using e.coli expression system. The N-terminal 6xHis tag and C-terminal 6xHis tag was smoothly integrated into the coding gene of eltB, which enables a simple process of detecting and purifying the eltB recombinant protein in the following steps.

The Escherichia coli heat-labile enterotoxin B chain (EltB) is a component of the heat-labile enterotoxin (LT) produced by certain strains of E. coli. LT is a major virulence factor associated with enterotoxigenic E. coli (ETEC), a pathotype known for causing traveler's diarrhea and infantile diarrhea in developing countries. EltB forms a heterohexameric complex with the A subunit (EltA), and together, they constitute the biologically active toxin. EltB is responsible for binding to host cell surface receptors, facilitating the internalization of the toxin into the intestinal epithelial cells. Once inside the cells, the A subunit exerts its toxic effects, leading to an increase in intracellular cyclic AMP (cAMP) levels, which disrupts ion transport and water balance, resulting in diarrhea. Understanding the structure and function of EltB is crucial for developing strategies to prevent ETEC infections.

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Target Background

Function
The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
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