Recombinant Escherichia coli Membrane protein insertase YidC (yidC), partial

1. We suggest that EH1 serves as a mechanical lever, facilitating a coordinated movement of YidC TMDs to trigger the release of nascent chains into the membrane. PMID: 27974208
2. The YidC prevents LacY from misfolding by stabilizing the unfolded state from which LacY inserts structural segments stepwise into the membrane until folding is completed. During stepwise insertion, YidC and the membrane together stabilize the transient folds. PMID: 27595331
3. Deletions in loop C1 affected the activity of YidC but did not influence ribosome binding or substrate insertion, whereas loop C2 was involved in ribosome binding. Combining the deletion with removal of the C terminus abolished YidC-mediated insertion. PMID: 26023232
4. These findings extend the function of YidC as a foldase for membrane protein (complexes) to periplasmic domains of membrane proteins. PMID: 25666136
5. Data indicate that the conserved positively charged residue was not required for the E. coli YidC or the A. thaliana Alb3 to functionally complement the E. coli YidC depletion strain or to promote insertion of YidC-dependent membrane proteins. PMID: 25359772
6. Conserved proline residues on the cytoplasmic side of conserved core transmembrane segments contribute to flexibility and structure of the protein YidC. PMID: 24801559
7. The YidC protein is critical for the insertion of a subset of proteins into membranes. PMID: 22108384
8. The data show the broad physiological contribution of YidC to the bacterial cell and the considerable ramification to the cell when it is depleted. PMID: 20061485
9. the Sec-independent function of YidC is conserved and essential for cell growth PMID: 15671040
10. Required for membrane insertion of F1F0 ATP synthase. PMID: 16880204
11. Both alleles impart a cold-sensitive phenotype and result from point mutations localized to the third transmembrane (TM3) segment of YidC, indicating that this region is crucial for YidC function. PMID: 17933892
12. they define the features of a membrane protein transmembrane segment that induce lateral release from the YidC and Sec translocases into the lipid bilayer in bacteria PMID: 18052199
13. The region corresponding to the C-terminal alpha-helix was previously shown to be important for the protein insertase function of YidC and is conserved in other YidC-like proteins. PMID: 18093969
14. YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex PMID: 18456666
15. Expression and purification of the recombinant membrane protein YidC PMID: 18586516
16. specific residues of the third YidC transmembrane segment alpha-helix is oriented toward the transmembrane domains of nascent inner membrane proteins that, in contrast, appear quite flexibly positioned at this stage in biogenesis PMID: 18840604
17. A detailed disulfide-mapping study revealed that one face of TM3 of YidC makes contact with the Pf3 protein. PMID: 18996118
18. Results suggest that dimers of the YidC and Oxa1 proteins form insertion pores and share a common overall architecture with the SecY monomer. PMID: 19450532

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KEGG: ecj:JW3683

STRING: 316385.ECDH10B_3892