Name: Recombinant Escherichia coli Stringent starvation protein B (sspB)
Brand: CUSABIO
SKU: 365382ENV

Product Details

Purity

>85% (SDS-PAGE)

Target Names

sspB

Uniprot No.

P0AFZ3

Alternative Names

sspB; b3228; JW3197; Stringent starvation protein B; Adapter protein SspB; Specificity-enhancing factor SspB

Species

Escherichia coli (strain K12)

Expression Region

1-165

Target Protein Sequence

MDLSQLTPRR PYLLRAFYEW LLDNQLTPHL VVDVTLPGVQ VPMEYARDGQ IVLNIAPRAV GNLELANDEV RFNARFGGIP RQVSVPLAAV LAIYARENGA GTMFEPEAAY DEDTSIMNDE EASADNETVM SVIDGDKPDH DDDTHPDDEP PQPPRGGRPA LRVVK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

Regarding your protein CSB-EP365382ENV, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information for all 4 expression hosts?
Also, have any customers reported any activity from this protein by any chance?
Thanks so much!

Thanks for your inquiry.
Recombinant Escherichia coli Stringent starvation protein B(sspB)
CSB-YP365382ENV >> Yeast
CSB-EP365382ENV >> E.coli
CSB-BP365382ENV >> Baculovirus
CSB-MP365382ENV >> Mammalian cell
Expression Region: 1-165aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows: EP, YP, BP, MP: N-terminal 6xHis-tagged.
Sequence:

MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK

Reference: http://www.uniprot.org/uniprot/P0AFZ3
Very nice to receive your inquiry.Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity, we can't 100% guarantee its activity.
However, as what we provide is the full length protein, and it's purifie under very mild condition, it should be active. In addition, this is an E. coli self protein
we recommend you to purchase a small size of EP protein to have a try, it's very likely to be active.
In addition, we would like to recommend the following protein, the EP protein has already been successfully expressed, and it's supernatant expression. It's very likely to be active.
The main difference between these two proteins is the species, however, their sequence similarity is 100%.
Recombinant Escherichia coli O157:H7 Stringent starvation protein B(sspB)
CSB-YP360347EOD >> Yeast
CSB-EP360347EOD >> E.coli
CSB-BP360347EOD >> Baculovirus
CSB-MP360347EOD >> Mammalian cell
Expression Region:1-165aa; Full length.
Tag information:EP: N-terminal 6xHis-tagged; YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for other expression system: YP, BP, MP:N-terminal 6xHis-tagged
Sequence:

MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK

Reference: http://www.uniprot.org/uniprot/P0AFZ4

Target Background

Function

Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.; Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.

Gene References into Functions

The describe an engineered split-adaptor system, in which sspB protein assembly and delivery of substrates to the ClpXP protease depends on a small molecule (rapamycin). PMID: 21866931
results also reveal highly conserved residues in the ClpX-binding peptides of both E. coli SspB and C. crescentus SspBalpha that play no detectable role in ClpX-binding or substrate delivery. PMID: 20014030
properties of the degradation tag and the number of SspB.ClpX tethering interactions are the major factors that determine the extent to which the substrate and ClpX are engaged in ternary delivery complexes PMID: 17317664
ClpXP, together with SspB, is uniquely adapted for degradation of SsrA-tagged proteins and is responsible for the major part of their degradation in vivo PMID: 18550539

Protein Families

SspB family

Database Links

KEGG: ecj:JW3197

STRING: 316385.ECDH10B_3405

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Code	CSB-YP365382ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP365382ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP365382ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP365382ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP365382ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Escherichia coli Stringent starvation protein B (sspB)

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